Source:http://linkedlifedata.com/resource/pubmed/id/16061685
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
15
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| pubmed:dateCreated |
2005-8-2
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| pubmed:abstractText |
Carcinoembryonic antigen (CEA) is a tumor-associated antigen targeted for the development of colorectal tumor vaccines. In this study, we developed papillomavirus pseudoviruses encoding the truncated CEA without NH2-terminal signal peptide (PV-CEA) as an oral vaccine to induce CEA-specific CTL responses. In CEA transgenic (CEA-Tg) mice orally immunized with PV-CEA, the immunologic tolerance to CEA as a "self-antigen" was overcome and both mucosal and systemic CEA-specific cytolytic activities were detected by in vitro 51Cr release assays. In a tumor prevention model, the growth rate of CEA+ tumors was significantly delayed in CEA-Tg mice orally immunized with PV-CEA when compared with the control vaccine. Further, the IFN-gamma enzyme-linked ImmunoSPOT and in vitro 51Cr release assay results showed that HLA-A2-restricted, CEA-specific CTL responses were induced in both mucosal and systemic lymphoid tissues in A2 transgenic mice after oral immunization with PV-CEA. Finally, we showed that coadministration of papillomavirus pseudoviruses encoding interleukin-2 with PV-CEA enhanced the generation of A2-restricted, CEA-specific CTLs in aged CEA/A2 double transgenic mice, which were more clinically relevant. Our data suggest that PV-CEA pseudovirus vaccine is a promising oral CEA vaccine for humans to induce CEA-specific CTLs at the site of colorectal tumors (i.e., intestinal mucosa), which might efficiently eliminate CEA+ colorectal tumor cells in the mucosa.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cancer Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Carcinoembryonic Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-A2 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Sorting Signals,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Vaccines
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0008-5472
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
65
|
| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
6990-9
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16061685-Adenocarcinoma,
pubmed-meshheading:16061685-Administration, Oral,
pubmed-meshheading:16061685-Animals,
pubmed-meshheading:16061685-Bovine papillomavirus 1,
pubmed-meshheading:16061685-Cancer Vaccines,
pubmed-meshheading:16061685-Carcinoembryonic Antigen,
pubmed-meshheading:16061685-Cell Line, Tumor,
pubmed-meshheading:16061685-Colorectal Neoplasms,
pubmed-meshheading:16061685-Female,
pubmed-meshheading:16061685-HLA-A2 Antigen,
pubmed-meshheading:16061685-Humans,
pubmed-meshheading:16061685-Immunity, Mucosal,
pubmed-meshheading:16061685-Male,
pubmed-meshheading:16061685-Mice,
pubmed-meshheading:16061685-Mice, Inbred C57BL,
pubmed-meshheading:16061685-Mice, Transgenic,
pubmed-meshheading:16061685-Peptide Fragments,
pubmed-meshheading:16061685-Plasmids,
pubmed-meshheading:16061685-Protein Sorting Signals,
pubmed-meshheading:16061685-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:16061685-Viral Vaccines
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| pubmed:year |
2005
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| pubmed:articleTitle |
Induction of mucosal and systemic immune responses against human carcinoembryonic antigen by an oral vaccine.
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| pubmed:affiliation |
Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, Illinois 60153, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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