Source:http://linkedlifedata.com/resource/pubmed/id/16052618
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
13
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| pubmed:dateCreated |
2005-8-22
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| pubmed:abstractText |
The proteome of a HUPO human serum reference sample was analyzed using multidimensional separation techniques at both the protein and the peptide levels. To eliminate false-positive identifications from the search results, we employed a data filtering method using molecular weight (MW) correlations derived from denaturing 1-DE. First, the six most abundant serum proteins were removed from the sample using immunoaffinity chromatography. 1-DE was then used to fractionate the remaining serum proteins according to the MW. Gel bands were isolated and in-gel digested with trypsin, and the resulting peptides were analyzed by 2-D LC/ESI-MS/MS. A SEQUEST search using the MS/MS results identified 494 proteins. Of these, 202 were excluded formally using protein data filtering as they were single-assignment proteins and their theoretical and electrophoretically-derived MWs did not correlate at high confidence. To evaluate this method, the results were compared with those of 1-D LC/MALDI-TOF/TOF and HUPO Plasma Proteome Project analyses. Our data filtering approach proved valuable in analysis of complex, large-scale proteomes such as human serum.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
|
| pubmed:issn |
1615-9853
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
5
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
3376-85
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16052618-Amino Acid Sequence,
pubmed-meshheading:16052618-Blood Proteins,
pubmed-meshheading:16052618-Chromatography, Affinity,
pubmed-meshheading:16052618-Chromatography, Liquid,
pubmed-meshheading:16052618-Databases, Protein,
pubmed-meshheading:16052618-Electrophoresis,
pubmed-meshheading:16052618-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:16052618-Humans,
pubmed-meshheading:16052618-Mass Spectrometry,
pubmed-meshheading:16052618-Molecular Sequence Data,
pubmed-meshheading:16052618-Molecular Weight,
pubmed-meshheading:16052618-Peptides,
pubmed-meshheading:16052618-Proteins,
pubmed-meshheading:16052618-Proteomics,
pubmed-meshheading:16052618-Reference Standards,
pubmed-meshheading:16052618-Spectrometry, Mass, Electrospray Ionization,
pubmed-meshheading:16052618-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:16052618-Statistics as Topic,
pubmed-meshheading:16052618-Trypsin
|
| pubmed:year |
2005
|
| pubmed:articleTitle |
Utility of electrophoretically derived protein mass estimates as additional constraints in proteome analysis of human serum based on MS/MS analysis.
|
| pubmed:affiliation |
Proteome Analysis Team, Korea Basic Science Institute, Yusung-Ku, Daejeon, Korea.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|