|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0001675,
umls-concept:C0006104,
umls-concept:C0017262,
umls-concept:C0185117,
umls-concept:C0205250,
umls-concept:C0458003,
umls-concept:C0475264,
umls-concept:C0678723,
umls-concept:C0851285,
umls-concept:C1171362,
umls-concept:C1332794,
umls-concept:C1515670,
umls-concept:C1540033,
umls-concept:C2911684
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2005-8-3
|
|
pubmed:abstractText |
Cytosolic tyrosine kinases play a critical role both in neural development and in adult brain function and plasticity. Here we isolated a cDNA with high homology to human Ack1 and mouse Tnk2. This cDNA directs the expression of a 125-kD protein that can be autophosphorylated in tyrosines. Initially, this clone was named Pyk1 for proline-rich tyrosine kinase (Lev et al., 1995); however, since it corresponds to the mouse homolog of Ack1, here we called it Ack1/Pyk1. In this study we show that Ack1/Pyk1 mRNA and protein is highly expressed in the developing and adult brain. The highest levels of Ack1/Pyk1 expression were detected in the hippocampus, neocortex, and cerebellum. Electron microscopy studies showed that Ack1/Pyk1 protein is expressed in these regions both at dendritic spines and presynaptic axon terminals, indicating a role in synaptic function. Furthermore, we demonstrate that Ack1/Pyk1 mRNA levels are strongly upregulated by increased neural activity, produced by intraperitoneal kainate injections. During development, Ack1/Pyk1 was also expressed in the proliferative ventricular zones and in postmitotic maturing neurons. In neuronal cultures, Ack1/Pyk1 was detected in developing dendrites and axons, including dendritic tips and growth cones. Moreover, Ack1/Pyk1 colocalized with Cdc42 GTPase in neuronal cultures and coimmunoprecipitated with Cdc42 in HEK 293T cells. Altogether, our findings indicate that Ack1/Pyk1 tyrosine kinase may be involved both in adult synaptic function and plasticity and in brain development.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Excitatory Amino Acid Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Glial Fibrillary Acidic Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Kainic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Microtubule-Associated Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mtap2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoamino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/TNK2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tnk2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tubulin,
http://linkedlifedata.com/resource/pubmed/chemical/beta3 tubulin, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/cdc42 GTP-Binding Protein
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0021-9967
|
|
pubmed:author |
pubmed-author:Casaroli-MaranoRicardoR,
pubmed-author:Del RioJosé AntonioJA,
pubmed-author:FontanaXavierX,
pubmed-author:FrancoNeusN,
pubmed-author:Ibáñez-SabioMiguel AngelMA,
pubmed-author:La TorreAnnaA,
pubmed-author:LowensteinEveE,
pubmed-author:MartínezAlbertA,
pubmed-author:PascualMartaM,
pubmed-author:SorianoEduardoE,
pubmed-author:UreñaJesús MarianoJM,
pubmed-author:Winsky-SommererRaphaelleR,
pubmed-author:de LeceaLuisL
|
|
pubmed:copyrightInfo |
(c) 2005 Wiley-Liss, Inc.
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
19
|
|
pubmed:volume |
490
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
119-32
|
|
pubmed:dateRevised |
2011-7-1
|
|
pubmed:meshHeading |
pubmed-meshheading:16052498-Animals,
pubmed-meshheading:16052498-Animals, Newborn,
pubmed-meshheading:16052498-Blotting, Northern,
pubmed-meshheading:16052498-Blotting, Western,
pubmed-meshheading:16052498-Brain,
pubmed-meshheading:16052498-Cells, Cultured,
pubmed-meshheading:16052498-Cloning, Molecular,
pubmed-meshheading:16052498-Embryo, Mammalian,
pubmed-meshheading:16052498-Excitatory Amino Acid Agonists,
pubmed-meshheading:16052498-Gene Expression Regulation, Developmental,
pubmed-meshheading:16052498-Glial Fibrillary Acidic Protein,
pubmed-meshheading:16052498-Humans,
pubmed-meshheading:16052498-Immunohistochemistry,
pubmed-meshheading:16052498-Immunoprecipitation,
pubmed-meshheading:16052498-In Situ Hybridization,
pubmed-meshheading:16052498-Kainic Acid,
pubmed-meshheading:16052498-Mice,
pubmed-meshheading:16052498-Microscopy, Immunoelectron,
pubmed-meshheading:16052498-Microtubule-Associated Proteins,
pubmed-meshheading:16052498-Neurons,
pubmed-meshheading:16052498-Phosphoamino Acids,
pubmed-meshheading:16052498-Phosphorylation,
pubmed-meshheading:16052498-Presynaptic Terminals,
pubmed-meshheading:16052498-Protein-Tyrosine Kinases,
pubmed-meshheading:16052498-RNA, Messenger,
pubmed-meshheading:16052498-Synapses,
pubmed-meshheading:16052498-Time Factors,
pubmed-meshheading:16052498-Tubulin,
pubmed-meshheading:16052498-cdc42 GTP-Binding Protein
|
|
pubmed:year |
2005
|
|
pubmed:articleTitle |
Expression, synaptic localization, and developmental regulation of Ack1/Pyk1, a cytoplasmic tyrosine kinase highly expressed in the developing and adult brain.
|
|
pubmed:affiliation |
Developmental Neurobiology and Regeneration Lab, Institute of Biomedical Research of Barcelona-Parc Científic de Barcelona, University of Barcelona, Josep Samitier 1-5, E08028 Barcelona, Spain. jurena@pcb.ub.es
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|
