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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2005-7-28
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pubmed:abstractText |
Allograft inflammatory factor (AIF)-1 is a cytoplasmic, calcium-binding protein whose expression in transplanted human hearts correlates with rejection and development of coronary artery vasculopathy (CAV). AIF-1 is constitutively expressed in monocytes/macrophages, but its expression in human lymphocytes has not been described. After immunohistochemical analysis of human coronary arteries with CAV, we identified AIF-1 expression in CD3-positive lymphocytes. AIF-1 was differentially expressed in peripheral blood mononuclear cells and in the T-lymphoblastoid MOLT-4 cell line exposed to various cytokines, suggesting a role for AIF-1 in T-lymphocyte activation. To determine AIF-1 function, MOLT-4 cells were stably transduced by AIF-1 retrovirus. Overexpression of AIF-1 in these cells led to a 238% increase in cell number compared to empty vector controls. AIF-1 polymerized nonmuscle actin and MOLT-4 cells overexpressing AIF-1 migrated 95% more rapidly than empty vector controls. Primary human vascular smooth muscle cells cultured in conditioned media from AIF-1-transduced MOLT-4 cells proliferated 99% more rapidly than vascular smooth muscle cells cultured in conditioned media from empty vector-transduced MOLT-4 cells. These data indicate that AIF-1 is expressed in activated T lymphocytes, that its expression enhances activation of lymphocytes, and that AIF-1 expression in activated lymphocytes may have important ramifications for activation of adjacent arterial vascular smooth muscle cells and development of CAV.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-10436187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-10564648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-10715441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-10894811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-11003350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-11322935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-11557666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-11722645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-11965043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-12368204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-12390951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-12492706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-12714565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-12941148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-14688331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-1892474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-2669279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-7586221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-7627715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-7769138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-7823872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-7900633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-8479518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-8545878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-8736690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-9323100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16049345-9928822
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0002-9440
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
167
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
619-26
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:16049345-Actins,
pubmed-meshheading:16049345-Arteriosclerosis,
pubmed-meshheading:16049345-Calcium-Binding Proteins,
pubmed-meshheading:16049345-Cell Movement,
pubmed-meshheading:16049345-Cell Proliferation,
pubmed-meshheading:16049345-Cells, Cultured,
pubmed-meshheading:16049345-Coronary Vessels,
pubmed-meshheading:16049345-Culture Media, Conditioned,
pubmed-meshheading:16049345-Cytokines,
pubmed-meshheading:16049345-DNA-Binding Proteins,
pubmed-meshheading:16049345-Humans,
pubmed-meshheading:16049345-Lymphocyte Activation,
pubmed-meshheading:16049345-Macrophages,
pubmed-meshheading:16049345-Muscle, Smooth, Vascular,
pubmed-meshheading:16049345-Myocardium,
pubmed-meshheading:16049345-Retroviridae,
pubmed-meshheading:16049345-T-Lymphocytes
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pubmed:year |
2005
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pubmed:articleTitle |
Expression of allograft inflammatory factor-1 in T lymphocytes: a role in T-lymphocyte activation and proliferative arteriopathies.
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pubmed:affiliation |
Department of Cardiology, Cardiovascular Research Center, Temple University School of Medicine, Philadelphia PA 19140, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, N.I.H., Extramural
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