Linked Life Data

16048945

Source:http://linkedlifedata.com/resource/pubmed/id/16048945

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2005-7-28
pubmed:abstractText
In this study, we investigated the pH dependency of the in vitro activities of amphotericin B (AMB) and flucytosine (5FC) against Candida spp., Cryptococcus neoformans, Aspergillus fumigatus, Rhizopus spp., and Scedosporium prolificans in RPMI 1640 buffered with citrate buffer (pH 4.0, 5.0, 5.4, and 6.0), citrate-phosphate buffer (pH 5.4, 6.0, 6.4, and 7.0), and 3-[N-morpholino]propanesulfonic acid (MOPS) (pH 6.4, 7.0, 7.4, and 7.9). For 5FC, no significant differences were found between MICs obtained with the different buffers, while for AMB, significant differences were found. The MICs obtained with citrate-phosphate buffer were approximately 1 twofold-dilution step higher than the MICs obtained with MOPS. We demonstrated that the in vitro activities of AMB and 5FC against yeast and mold isolates were pH dependent. The in vitro activity of AMB decreased when the pH was lowered, while the in vitro activity of 5FC increased. The effect of the pH on the in vitro activities was dependent not only on the antifungal agent tested but also on the microorganism. For AMB, there was a nonlinear relationship (median r(2), 0.864) for Candida spp., C. neoformans, A. fumigatus, and Rhizopus spp. over the pH range tested. The mean MICs ranged from 0.5 to 2.52 microg/ml at pH 7.0 and from 20.16 to 32 microg/ml at pH 5.0. For S. prolificans, there was no relationship. For 5FC, there was a linear relationship for Candida spp. (median r(2), 0.767) and a nonlinear relationship for C. neoformans and A. fumigatus (median r(2), 0.882) over the pH range tested. The mean MIC values ranged from 0.125 to 1,024 microg/ml at pH 7.0 and from 0.02 to 4 microg/ml at pH 5.0. For Rhizopus spp. and S. prolificans, the relationship could not be determined, since the MIC was >1,024 microg/ml over a pH range of 4.0 to 7.9.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16048945-11585779, http://linkedlifedata.com/resource/pubmed/commentcorrection/16048945-11724829, http://linkedlifedata.com/resource/pubmed/commentcorrection/16048945-12797387, http://linkedlifedata.com/resource/pubmed/commentcorrection/16048945-15273136, http://linkedlifedata.com/resource/pubmed/commentcorrection/16048945-1998406, http://linkedlifedata.com/resource/pubmed/commentcorrection/16048945-2467987, http://linkedlifedata.com/resource/pubmed/commentcorrection/16048945-6094418, http://linkedlifedata.com/resource/pubmed/commentcorrection/16048945-6969281, http://linkedlifedata.com/resource/pubmed/commentcorrection/16048945-6993368, http://linkedlifedata.com/resource/pubmed/commentcorrection/16048945-9061353, http://linkedlifedata.com/resource/pubmed/commentcorrection/16048945-9564569, http://linkedlifedata.com/resource/pubmed/commentcorrection/16048945-9738841
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0066-4804
pubmed:author
pubmed:issnType
Print
pubmed:volume
49
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3341-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Relationship between in vitro activities of amphotericin B and flucytosine and pH for clinical yeast and mold isolates.
pubmed:affiliation
Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Weg door Jonkerbos 100, 6532 SZ Nijmegen, The Netherlands.
pubmed:publicationType
Journal Article, Evaluation Studies