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pubmed-article:16048323rdf:typepubmed:Citationlld:pubmed
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pubmed-article:16048323pubmed:dateCreated2005-7-28lld:pubmed
pubmed-article:16048323pubmed:abstractTextA new solution-phase phosphoramidite approach is reported for oligonucleotide synthesis employing recyclable solid-supported reagents. It uses polyvinyl pyridinium tosylate as the activator of a nucleoside-3'-O-phosphoramidite in the coupling step with a 5'-OH nucleoside or dinucleotide. The resulting phosphite triester was either sulfurized or oxidized using polystyrene-bound trimethylammonium tetrathionate or periodiate. This method avoids complicated purification steps, as excess reagents are easily removed by filtration. [reaction: see text]lld:pubmed
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pubmed-article:16048323pubmed:authorpubmed-author:ImbachJean-Lo...lld:pubmed
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pubmed-article:16048323pubmed:authorpubmed-author:LangeMeinolfMlld:pubmed
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pubmed-article:16048323pubmed:pagination3485-8lld:pubmed
pubmed-article:16048323pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:16048323pubmed:year2005lld:pubmed
pubmed-article:16048323pubmed:articleTitleHigh-yield solution-phase synthesis of di- and trinucleotide blocks assisted by polymer-supported reagents.lld:pubmed
pubmed-article:16048323pubmed:affiliationERT Oligonucléotides: Méthodologie, Valorisation, UMR 5625 CNRS-UM2 University Montpellier II, 34095 Montpellier, France.lld:pubmed
pubmed-article:16048323pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:16048323pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed