Linked Life Data

16048323

Source:http://linkedlifedata.com/resource/pubmed/id/16048323

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
2005-7-28
pubmed:abstractText
A new solution-phase phosphoramidite approach is reported for oligonucleotide synthesis employing recyclable solid-supported reagents. It uses polyvinyl pyridinium tosylate as the activator of a nucleoside-3'-O-phosphoramidite in the coupling step with a 5'-OH nucleoside or dinucleotide. The resulting phosphite triester was either sulfurized or oxidized using polystyrene-bound trimethylammonium tetrathionate or periodiate. This method avoids complicated purification steps, as excess reagents are easily removed by filtration. [reaction: see text]
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1523-7060
pubmed:author
pubmed:issnType
Print
pubmed:day
4
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3485-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
High-yield solution-phase synthesis of di- and trinucleotide blocks assisted by polymer-supported reagents.
pubmed:affiliation
ERT Oligonucléotides: Méthodologie, Valorisation, UMR 5625 CNRS-UM2 University Montpellier II, 34095 Montpellier, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't