|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2005-7-27
|
|
pubmed:abstractText |
Both viral effect and immune-mediated mechanism are involved in the pathogenesis of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infection. In this study, we showed that in SARS patient sera there were autoantibodies (autoAbs) that reacted with A549 cells, the type-2 pneumocytes, and that these autoAbs were mainly IgG. The autoAbs were detectable 20 days after fever onset. Tests of non-SARS-pneumonia patients did not show the same autoAb production as in SARS patients. After sera IgG bound to A549 cells, cytotoxicity was induced. Cell cytotoxicity and the anti-epithelial cell IgG level were positively correlated. Preabsorption and binding assays indicated the existence of cross-reactive epitopes on SARS-CoV spike protein domain 2 (S2). Furthermore, treatment of A549 cells with anti-S2 Abs and IFN-gamma resulted in an increase in the adherence of human peripheral blood mononuclear cells to these epithelial cells. Taken together, we have demonstrated that the anti-S2 Abs in SARS patient sera cause cytotoxic injury as well as enhance immune cell adhesion to epithelial cells. The onset of autoimmune responses in SARS-CoV infection may be implicated in SARS pathogenesis.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-10450521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-10896942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-11465101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-11549879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-12097367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-12436482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-12671061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-12690091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-12690092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-12692850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-12711465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-12730500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-12730501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-12747883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-12781535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-12781536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-12782660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-12892955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-14519257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-14631105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-14647384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-14730215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-14767818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-15008980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-15016880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-15018126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-15018132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-15024391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-15030507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-15030702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-15078936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-15200814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-15602737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-1702393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-1848306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-1991445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-2300583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-8397257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16045740-9024155
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0009-9104
|
|
pubmed:author |
pubmed-author:FangY TYT,
pubmed-author:KuoY MYM,
pubmed-author:LEEM MMM,
pubmed-author:LeiH YHY,
pubmed-author:LiaoP CPC,
pubmed-author:LiuY LYL,
pubmed-author:MinB HBH,
pubmed-author:RaoM PMP,
pubmed-author:ShiehC C KCC,
pubmed-author:SuI JIJ,
pubmed-author:WangH JHJ,
pubmed-author:YenJ HJH
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
141
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
500-8
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:16045740-Autoantibodies,
pubmed-meshheading:16045740-Cell Adhesion,
pubmed-meshheading:16045740-Cell Death,
pubmed-meshheading:16045740-Cell Line, Tumor,
pubmed-meshheading:16045740-Cross Reactions,
pubmed-meshheading:16045740-Cytotoxicity Tests, Immunologic,
pubmed-meshheading:16045740-Epithelial Cells,
pubmed-meshheading:16045740-Humans,
pubmed-meshheading:16045740-Immunoglobulin G,
pubmed-meshheading:16045740-Lung,
pubmed-meshheading:16045740-Membrane Glycoproteins,
pubmed-meshheading:16045740-SARS Virus,
pubmed-meshheading:16045740-Severe Acute Respiratory Syndrome,
pubmed-meshheading:16045740-Viral Envelope Proteins
|
|
pubmed:year |
2005
|
|
pubmed:articleTitle |
Antibody to severe acute respiratory syndrome (SARS)-associated coronavirus spike protein domain 2 cross-reacts with lung epithelial cells and causes cytotoxicity.
|
|
pubmed:affiliation |
Department of Microbiology and Immunology, National Cheng Kung University Medical College, Tainan, Taiwan.
|
|
pubmed:publicationType |
Journal Article
|
