Source:http://linkedlifedata.com/resource/pubmed/id/16042405
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
30
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| pubmed:dateCreated |
2005-7-26
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| pubmed:abstractText |
Although studies in vitro and in yeast suggest that acyl-CoA binding protein ACBP may modulate long-chain fatty acyl-CoA (LCFA-CoA) distribution, its physiological function in mammals is unresolved. To address this issue, the effect of ACBP on liver LCFA-CoA pool size, acyl chain composition, distribution, and transacylation into more complex lipids was examined in transgenic mice expressing a higher level of ACBP. While ACBP transgenic mice did not exhibit altered body or liver weight, liver LCFA-CoA pool size increased by 69%, preferentially in saturated and polyunsaturated, but not monounsaturated, LCFA-CoAs. Intracellular LCFA-CoA distribution was also altered such that the ratio of LCFA-CoA content in (membranes, organelles)/cytosol increased 2.7-fold, especially in microsomes but not mitochondria. The increased distribution of specific LCFA-CoAs to the membrane/organelle and microsomal fractions followed the same order as the relative LCFA-CoA binding affinity exhibited by murine recombinant ACBP: saturated > monounsaturated > polyunsaturated C14-C22 LCFA-CoAs. Consistent with the altered microsomal LCFA-CoA level and distribution, enzymatic activity of liver microsomal glycerol-3-phosphate acyltransferase (GPAT) increased 4-fold, liver mass of phospholipid and triacylglyceride increased nearly 2-fold, and relative content of monounsaturated C18:1 fatty acid increased 44% in liver phospholipids. These effects were not due to the ACBP transgene altering the protein levels of liver microsomal acyltransferase enzymes such as GPAT, lysophosphatidic acid acyltransferase (LAT), or acyl-CoA cholesterol acyltransferase 2 (ACAT-2). Thus, these data show for the first time in a physiological context that ACBP expression may play a role in LCFA-CoA metabolism.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acyl Coenzyme A,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Diazepam Binding Inhibitor,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acid-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Glycerol-3-Phosphate...,
http://linkedlifedata.com/resource/pubmed/chemical/Glycolipids,
http://linkedlifedata.com/resource/pubmed/chemical/Lipids,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipids,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sterol O-Acyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Triglycerides,
http://linkedlifedata.com/resource/pubmed/chemical/glycerolglycolipids,
http://linkedlifedata.com/resource/pubmed/chemical/sterol O-acyltransferase 2
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0006-2960
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
2
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| pubmed:volume |
44
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
10282-97
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:16042405-Acyl Coenzyme A,
pubmed-meshheading:16042405-Animals,
pubmed-meshheading:16042405-Body Weight,
pubmed-meshheading:16042405-Carrier Proteins,
pubmed-meshheading:16042405-Diazepam Binding Inhibitor,
pubmed-meshheading:16042405-Fatty Acid-Binding Proteins,
pubmed-meshheading:16042405-Fatty Acids,
pubmed-meshheading:16042405-Glycerol-3-Phosphate O-Acyltransferase,
pubmed-meshheading:16042405-Glycolipids,
pubmed-meshheading:16042405-Lipid Metabolism,
pubmed-meshheading:16042405-Lipids,
pubmed-meshheading:16042405-Liver,
pubmed-meshheading:16042405-Mice,
pubmed-meshheading:16042405-Mice, Transgenic,
pubmed-meshheading:16042405-Organ Size,
pubmed-meshheading:16042405-Phospholipids,
pubmed-meshheading:16042405-Protein Binding,
pubmed-meshheading:16042405-Recombinant Proteins,
pubmed-meshheading:16042405-Sterol O-Acyltransferase,
pubmed-meshheading:16042405-Triglycerides
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| pubmed:year |
2005
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| pubmed:articleTitle |
Acyl-coenzyme A binding protein expression alters liver fatty acyl-coenzyme A metabolism.
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| pubmed:affiliation |
Department of Physiology and Pharmacology, Texas A&M University, College Station, Texas 77843-4466, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, N.I.H., Extramural
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