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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
30
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pubmed:dateCreated |
2005-7-26
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pubmed:databankReference |
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pubmed:abstractText |
Cysteine-rich secretory proteins (CRISPs) are widespread in snake venoms. Some members of these CRISPs recently have been found to block L-type Ca(2+) channels or cyclic nucleotide-gated ion (CNG) channels. Here, natrin purified from Naja atra venom, a member of the CRISP family, can induce a further contractile response in the endothelium-denuded thoracic aorta of mouse which has been contracted by a high-K(+) solution. Further experiments show it can block the high-conductance calcium-activated potassium (BK(Ca)) channel in a concentration-dependent manner with an IC(50) of 34.4 nM and a Hill coefficient of 1.02, which suggests that only a single natrin molecule is required to bind an ion channel to block BK(Ca) current. The crystal structure of natrin displaying two domains in tandem shows its cysteine-rich domain (CRD) has relatively independent flexibility, especially for the C-terminal long loop (loop I) of CRD to participate in the interface of two domains. On the basis of previous studies of CNG channel and L-Ca(2+) channel blockers, and the sequence and structural comparison of natrin and stecrisp, the deviation of the vital loop I of CRD is suggested to contribute to different effects of some CRISPs in protein-protein interaction.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cobra Venoms,
http://linkedlifedata.com/resource/pubmed/chemical/Crisp1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Crotalid Venoms,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels...,
http://linkedlifedata.com/resource/pubmed/chemical/Solutions,
http://linkedlifedata.com/resource/pubmed/chemical/natrin protein, Naja atra,
http://linkedlifedata.com/resource/pubmed/chemical/stecrisp protein, Trimeresurus...
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-2960
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pubmed:author |
pubmed-author:ChengXin PingXP,
pubmed-author:DuanYuanyuanY,
pubmed-author:HaoQuanQ,
pubmed-author:HuangQingqiuQ,
pubmed-author:LouXiaohuaX,
pubmed-author:MoeJ FJF,
pubmed-author:NiuLiwenL,
pubmed-author:OKAM JMJ,
pubmed-author:ShenBingB,
pubmed-author:TengMaikunM,
pubmed-author:WangJingJ
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pubmed:issnType |
Print
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pubmed:day |
2
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pubmed:volume |
44
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10145-52
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16042391-Amino Acid Sequence,
pubmed-meshheading:16042391-Animals,
pubmed-meshheading:16042391-Aorta, Thoracic,
pubmed-meshheading:16042391-Cobra Venoms,
pubmed-meshheading:16042391-Crotalid Venoms,
pubmed-meshheading:16042391-Crystallography, X-Ray,
pubmed-meshheading:16042391-Cysteine,
pubmed-meshheading:16042391-Dose-Response Relationship, Drug,
pubmed-meshheading:16042391-Ion Channel Gating,
pubmed-meshheading:16042391-Male,
pubmed-meshheading:16042391-Membrane Glycoproteins,
pubmed-meshheading:16042391-Mice,
pubmed-meshheading:16042391-Models, Chemical,
pubmed-meshheading:16042391-Models, Molecular,
pubmed-meshheading:16042391-Molecular Sequence Data,
pubmed-meshheading:16042391-Muscle, Smooth, Vascular,
pubmed-meshheading:16042391-Muscle Contraction,
pubmed-meshheading:16042391-Potassium Channel Blockers,
pubmed-meshheading:16042391-Potassium Channels, Calcium-Activated,
pubmed-meshheading:16042391-Solutions
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pubmed:year |
2005
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pubmed:articleTitle |
Blocking effect and crystal structure of natrin toxin, a cysteine-rich secretory protein from Naja atra venom that targets the BKCa channel.
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pubmed:affiliation |
Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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