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rdf:type |
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lifeskim:mentions |
umls-concept:C0032105,
umls-concept:C0033684,
umls-concept:C0037293,
umls-concept:C0181496,
umls-concept:C0205314,
umls-concept:C0392747,
umls-concept:C0443172,
umls-concept:C0679199,
umls-concept:C0679622,
umls-concept:C0682260,
umls-concept:C0936012,
umls-concept:C1510827,
umls-concept:C1511726,
umls-concept:C1522485,
umls-concept:C2347074
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pubmed:issue |
12
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pubmed:dateCreated |
2005-8-10
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pubmed:abstractText |
A novel strategy consisting of cleavable Isotope-Coded Affinity Tag (cICAT) combined with MASCOT Distiller was evaluated as a tool for the quantification of proteins in "abnormal" patient plasma, prepared by pooling samples from patients with acute stroke. Quantification of all light and heavy cICAT-labelled peptide ion pairs was obtained using MASCOT Distiller combined with a proprietary software. Peptides displaying differences were selected for identification by MS. These preliminary results show the promise of our approach to identify potential biomarkers.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1615-9853
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3040-4
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16041675-Algorithms,
pubmed-meshheading:16041675-Animals,
pubmed-meshheading:16041675-Biological Markers,
pubmed-meshheading:16041675-Blood Proteins,
pubmed-meshheading:16041675-Computational Biology,
pubmed-meshheading:16041675-Databases, Protein,
pubmed-meshheading:16041675-Humans,
pubmed-meshheading:16041675-Immunoglobulin G,
pubmed-meshheading:16041675-Ions,
pubmed-meshheading:16041675-Isotopes,
pubmed-meshheading:16041675-Mass Spectrometry,
pubmed-meshheading:16041675-Peptide Mapping,
pubmed-meshheading:16041675-Peptides,
pubmed-meshheading:16041675-Proteins,
pubmed-meshheading:16041675-Proteome,
pubmed-meshheading:16041675-Proteomics,
pubmed-meshheading:16041675-Software,
pubmed-meshheading:16041675-Time Factors,
pubmed-meshheading:16041675-Up-Regulation
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pubmed:year |
2005
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pubmed:articleTitle |
A novel strategy using MASCOT Distiller for analysis of cleavable isotope-coded affinity tag data to quantify protein changes in plasma.
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pubmed:affiliation |
Proteome Sciences plc, South Wing Laboratory, Institute of Psychiatry, London, UK. kit-yi.leung@proteomics.com
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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