Source:http://linkedlifedata.com/resource/pubmed/id/16041150
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
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| pubmed:dateCreated |
2005-7-25
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| pubmed:abstractText |
The method of electrophoretic mobility shift assay under high-pressure conditions was improved using a high-pressure electrophoresis apparatus with capillary narrow-tube gel. It was found that the protein-DNA complex in the gel was stained as a high-resolution spot with ethidium bromide. Using this method, it was found that the behavior under high-pressure conditions of the protein-DNA complex composed of NtrC protein and its target promoter DNA is important for the pressure-regulated transcription process, and it was confirmed that the complex was dissociated above a pressure of 70 MPa.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
|
| pubmed:issn |
0916-8451
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
69
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1415-7
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| pubmed:meshHeading |
pubmed-meshheading:16041150-Atmospheric Pressure,
pubmed-meshheading:16041150-Bacterial Proteins,
pubmed-meshheading:16041150-DNA, Bacterial,
pubmed-meshheading:16041150-DNA-Binding Proteins,
pubmed-meshheading:16041150-Electrophoresis, Capillary,
pubmed-meshheading:16041150-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:16041150-Protein Binding,
pubmed-meshheading:16041150-Reproducibility of Results,
pubmed-meshheading:16041150-Shewanella
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| pubmed:year |
2005
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| pubmed:articleTitle |
Protein-DNA interactions under high-pressure conditions, studied by capillary narrow-tube electrophoresis.
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| pubmed:affiliation |
Extremobiosphere Research Center (XBR), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan. kawanoh@jamstec.go.jp
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| pubmed:publicationType |
Journal Article
|