|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
8
|
|
pubmed:dateCreated |
2005-7-25
|
|
pubmed:abstractText |
Listeria monocytogenes is a gram-positive intracellular pathogen that can enter phagocytic and nonphagocytic cells and colonize their cytosols. Taking advantage of this property to generate an intracellular vaccine delivery vector, we previously described a mutant strain of L. monocytogenes, Deltadal Deltadat, which is unable to synthesize cell wall by virtue of deletions in two genes (dal and dat) required for d-alanine synthesis. This highly attenuated strain induced long-lived protective systemic and mucosal immune responses in mice when administered in the transient presence of d-alanine. We have now increased the usefulness of this organism as a vaccine vector by use of an inducible complementation system that obviates the need for exogenous d-alanine administration. The strain expresses a copy of the Bacillus subtilis racemase gene under the control of a tightly regulated isopropyl-beta-d-thiogalactopyranoside (IPTG)-inducible promoter present on a multicopy plasmid. This bacterium demonstrates strict dose-dependent growth in the presence of IPTG. After removal of inducer, bacterial growth ceased within two replication cycles. Following infection of mice in the absence of IPTG or d-alanine, the bacterium survived in vivo for less than 3 days. Nevertheless, a single immunization elicited a state of long-lasting protective immunity against wild-type L. monocytogenes and induced a subset of effector listeriolysin O-specific CD11a(+) CD8(+) T cells in spleen and other tissues that was strongly enhanced after secondary immunization. This improved L. monocytogenes vector system may have potential use as a live vaccine against human immunodeficiency virus, other infectious diseases, and cancer.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-10493175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-11120806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-11222702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-11257401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-11517630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-11679669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-11714814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-11849532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-12065500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-12374827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-1369298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-1400255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-14467923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-15365184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-15385459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-1548084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-1607662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-1686033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-1689787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-1891356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-1905979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-1965218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-2147195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-2172168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-2443444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-2492614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-2509366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-2833557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-3007363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-3029033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-3926758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-6420789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-6811573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-7547706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-7585097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-7591052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-7591098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-7594479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-7732018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-8045263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-8127896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-8300212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-8344365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-8476559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-8627149,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-8902392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-9069289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-9141215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-9314084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-9343203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-9491894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16041022-9673233
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0019-9567
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
73
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
5065-73
|
|
pubmed:dateRevised |
2010-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:16041022-Alanine Racemase,
pubmed-meshheading:16041022-Animals,
pubmed-meshheading:16041022-Bacterial Vaccines,
pubmed-meshheading:16041022-CD8-Positive T-Lymphocytes,
pubmed-meshheading:16041022-Fibroblasts,
pubmed-meshheading:16041022-Immunity, Cellular,
pubmed-meshheading:16041022-Listeria monocytogenes,
pubmed-meshheading:16041022-Listeriosis,
pubmed-meshheading:16041022-Mice,
pubmed-meshheading:16041022-Mutation
|
|
pubmed:year |
2005
|
|
pubmed:articleTitle |
Conditional lethality yields a new vaccine strain of Listeria monocytogenes for the induction of cell-mediated immunity.
|
|
pubmed:affiliation |
203C Johnson Pavilion, 3610 Hamilton Walk, Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, N.I.H., Extramural
|
