Linked Life Data

16040607

Source:http://linkedlifedata.com/resource/pubmed/id/16040607

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
37
pubmed:dateCreated
2005-9-12
pubmed:databankReference
pubmed:abstractText
Fruiting body development in Myxococcus xanthus is a multicellular event that is coordinated by exchanging intercellular signals. FruA is a transcription factor essential for fruiting body development and is thought to play a key role in the C-signal pathway. Here we present the first identification of a gene regulated by FruA. The gene was isolated from a genomic library via in vitro selection in a DNA binding assay by using the DNA-binding domain of FruA tagged with His(8) at the C-terminal end (FruA-DBD-H(8)). The gene, named fdgA (FruA-dependent gene A), encodes a protein homologous to the outer-membrane auxiliary family protein involved in the polysaccharide export system. FruA-DBD-H(8) bound the upstream promoter region of the fdgA gene from nucleotide -89 to nucleotide -64 with respect to the transcription initiation site, which was required for the induction of fdgA expression during development. fdgA mRNA induced during development was absent in a fruA deletion strain. The deletion of fdgA resulted in defective fruiting body formation and reduced sporulation efficiency (1% that of the parent strain). Moreover, FruA was required for the developmental expression of sasA, which is also involved in the biosynthesis of the lipopolysaccharide O-antigen and is required for fruiting body development. Furthermore, the expression of both fdgA and sasA was partially dependent on the C-signal. These findings expand our understanding of the signal transduction pathway mediated by FruA during development in M. xanthus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
280
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
32279-84
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16040607-Amino Acid Sequence, pubmed-meshheading:16040607-Bacterial Proteins, pubmed-meshheading:16040607-Base Sequence, pubmed-meshheading:16040607-Binding Sites, pubmed-meshheading:16040607-Biological Transport, pubmed-meshheading:16040607-DNA, pubmed-meshheading:16040607-Gene Deletion, pubmed-meshheading:16040607-Gene Library, pubmed-meshheading:16040607-Genome, pubmed-meshheading:16040607-Lac Operon, pubmed-meshheading:16040607-Lipopolysaccharides, pubmed-meshheading:16040607-Molecular Sequence Data, pubmed-meshheading:16040607-Mutation, pubmed-meshheading:16040607-Myxococcus xanthus, pubmed-meshheading:16040607-Phenotype, pubmed-meshheading:16040607-Polysaccharides, pubmed-meshheading:16040607-Protein Binding, pubmed-meshheading:16040607-Protein Structure, Tertiary, pubmed-meshheading:16040607-RNA, Messenger, pubmed-meshheading:16040607-Signal Transduction, pubmed-meshheading:16040607-Transcription, Genetic, pubmed-meshheading:16040607-Transcription Factors
pubmed:year
2005
pubmed:articleTitle
Identification of a gene involved in polysaccharide export as a transcription target of FruA, an essential factor for Myxococcus xanthus development.
pubmed:affiliation
Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't