Linked Life Data

16037947

Source:http://linkedlifedata.com/resource/pubmed/id/16037947

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-7-27
pubmed:abstractText
The search for alternative sources of dopaminergic cells, other than primary fetal tissue for transplantation in Parkinson's disease has become a major focus of research. Different methodological approaches have led to generation in vitro of cells expressing DA-cell markers, although these cells are frequently unable to survive for a long time in vivo after transplantation and/or induce functional effects in the host brain. In the present study, we grafted cell aggregates treated with antibodies against fibroblast growth factor 4 into dopaminergic-denervated striata in rats. Furthermore, we grafted cell suspensions from primary mesencephalic fetal tissue. Grafts from expanded precursors were able to survive (at least 3 months postgrafting) and most decreased the lesion-induced ipsiversive rotation. In addition, immunolabeling for tyrosine hydroxylase and/or Fos showed that the grafts reinnervated the surrounding striatal tissue with dopaminergic terminals, and induced the expression of Fos in the striatal neurons of the reinnervated area after administration of amphetamine to the host rat. The number of dopaminergic cells in grafts from expanded precursors inducing rotational recovery was usually lower (1,226+/-314) than that in grafts from primary fetal tissue (1,671+/-122), but they were more densely packed in grafts that were of smaller volume and did not have the characteristic central nondopaminergic area observed in grafts from primary fetal tissue. The results suggest that long-term survival and functional integration into the DA-denervated striatum can be achieved with grafts of expanded mesencephalic precursors.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0887-4476
pubmed:author
pubmed:copyrightInfo
Synapse 58:12-21, 2005. (c) 2005 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
13-22
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16037947-Adrenergic Agents, pubmed-meshheading:16037947-Animals, pubmed-meshheading:16037947-Aromatic-L-Amino-Acid Decarboxylases, pubmed-meshheading:16037947-Behavior, Animal, pubmed-meshheading:16037947-Blotting, Western, pubmed-meshheading:16037947-Cell Transplantation, pubmed-meshheading:16037947-Corpus Striatum, pubmed-meshheading:16037947-Dopamine, pubmed-meshheading:16037947-Dopamine Plasma Membrane Transport Proteins, pubmed-meshheading:16037947-Female, pubmed-meshheading:16037947-Fibroblast Growth Factor 4, pubmed-meshheading:16037947-Immunohistochemistry, pubmed-meshheading:16037947-Motor Activity, pubmed-meshheading:16037947-Nerve Regeneration, pubmed-meshheading:16037947-Neurons, pubmed-meshheading:16037947-Oxidopamine, pubmed-meshheading:16037947-Rats, pubmed-meshheading:16037947-Rats, Sprague-Dawley, pubmed-meshheading:16037947-Recovery of Function, pubmed-meshheading:16037947-Serotonin, pubmed-meshheading:16037947-Tyrosine 3-Monooxygenase
pubmed:year
2005
pubmed:articleTitle
Expanded mesencephalic precursors develop into grafts of densely packed dopaminergic neurons that reinnervate the surrounding striatum and induce functional responses in the striatal neurons.
pubmed:affiliation
Laboratory of Neuroanatomy and Experimental Neurology, Department of Morphological Sciences, Faculty of Medicine, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't