Source:http://linkedlifedata.com/resource/pubmed/id/16034592
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| Predicate | Object |
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| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2005-12-1
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| pubmed:abstractText |
Fructans play important roles not only as a carbon source for survival under persistent snow cover but also as agents that protect against various stresses in overwintering plants. Complex fructans having both beta-(2,1)- and beta-(2,6)-linked fructosyl units accumulate in wheat (Triticum aestivum L.) during cold hardening. We detected fructan: fructan 1-fructosyltransferase (1-FFT; EC 2.4.1.100) activity for catalyzing the formation and extension of beta-(2,1)-linked fructans in hardened wheat tissues, cloned cDNAs (wft3 and wft4) of 1-FFT, and analyzed the enzymatic properties of a wft3 recombinant protein (Wft3m) produced by yeast. Wft3m transferred beta-(2,1)-linked fructosyl units to phlein, an extension of sucrose through beta-(2,6)-linked fructosyl units, as well as to inulin, an extension of sucrose through beta-(2,1)-linked fructosyl units, but could not efficiently synthesize long inulin oligomers. Incubation of a mixture of Wft3m and another recombinant protein of wheat, sucrose:fructan 6-fructosyltransferase (6-SFT), with sucrose and 1-kestotriose produced fructans similar to those that accumulated in hardened wheat tissues. The results demonstrate that 1-FFT produces branches of beta-(2,1)-linked fructosyl units to phlein and graminan oligomers synthesized by 6-SFT and contributes to accumulation of fructans containing beta-(2,1)- and beta-(2,6)-linked fructosyl units. In combination with sucrose:sucrose 1-fructosyltransferase (1-SST; EC 2.4.1.99) and 6-SFT, 1-FFT is necessary for fructan synthesis in hardened wheat.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1,2-beta-fructan...,
http://linkedlifedata.com/resource/pubmed/chemical/Fructans,
http://linkedlifedata.com/resource/pubmed/chemical/Hexosyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/sucrose - fructan...
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| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
0032-0935
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
223
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
90-104
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| pubmed:meshHeading |
pubmed-meshheading:16034592-Amino Acid Sequence,
pubmed-meshheading:16034592-Carbohydrate Conformation,
pubmed-meshheading:16034592-Carbohydrate Metabolism,
pubmed-meshheading:16034592-Chromatography, Ion Exchange,
pubmed-meshheading:16034592-Cloning, Molecular,
pubmed-meshheading:16034592-Cold Climate,
pubmed-meshheading:16034592-Fructans,
pubmed-meshheading:16034592-Gene Library,
pubmed-meshheading:16034592-Hexosyltransferases,
pubmed-meshheading:16034592-Molecular Sequence Data,
pubmed-meshheading:16034592-Pichia,
pubmed-meshheading:16034592-RNA, Messenger,
pubmed-meshheading:16034592-Recombinant Fusion Proteins,
pubmed-meshheading:16034592-Seasons,
pubmed-meshheading:16034592-Sequence Alignment,
pubmed-meshheading:16034592-Solubility,
pubmed-meshheading:16034592-Transfection,
pubmed-meshheading:16034592-Triticum
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| pubmed:year |
2005
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| pubmed:articleTitle |
Fructan:fructan 1-fructosyltransferase, a key enzyme for biosynthesis of graminan oligomers in hardened wheat.
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| pubmed:affiliation |
Laboratory of Fungal Disease, Department of Plant Pathology, National Agricultural Research Center, Kannondai, Tsukuba, Ibaraki 305-8666, Japan. akirak@affrc.go.jp
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| pubmed:publicationType |
Journal Article
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