Linked Life Data

16034202

Source:http://linkedlifedata.com/resource/pubmed/id/16034202

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2005-7-21
pubmed:abstractText
Quorum sensing is known to be related to regulation of virulence factors in some pathogens. We report transcriptional analysis of cdt genes (cdtA, B, and C ) encoding cytolethal distending toxin (CDT) in Campylobacter jejuni and demonstrate that a luxS mutation affects the cdt transcription in C. jejuni. The reverse transcriptase-polymerase chain reaction (RT-PCR) showed that cdtA, cdtB, and cdtC genes constitute a polycistronic operon in C. jejuni. A putative transcriptional start site was found at 81-bp upstream from the start codon of cdtA by the primer extension assay. A decrease in cdt transcription was observed in the luxS null mutant, which is defective in AI-2 production. The level of cdt transcription in the luxS mutant was approximately 61% of that in the wild-type. Flow cytometric analysis showed that the number of HeLa cells arrested in the G (2) /M phase, a common biological feature of CDT activity, was reduced by treatment with the cell-free supernatant of the luxS mutant as compared to that of the wild-type. Our observations suggest that the function of luxS is related to the regulation of cdt in C. jejuni.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0385-5600
pubmed:author
pubmed:issnType
Print
pubmed:volume
49
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
599-603
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Promoter analysis of cytolethal distending toxin genes (cdtA , B, and C ) and effect of a luxS mutation on CDT production in Campylobacter jejuni.
pubmed:affiliation
Laboratory of Veterinary Public Health, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
pubmed:publicationType
Journal Article