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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2005-7-21
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pubmed:abstractText |
HLA-E is an MHC class Ib molecule that binds nonamer peptides derived from the leader sequence of MHC class 1a molecules and is the major ligand for CD94/NKG2 receptors found on NK and T cells. Using the MHC class Ia-null cell line 721.221, we find that surface HLA-E increases following heat shock at 42 degrees C and NK cell-mediated lysis is inhibited using heat-stressed 721.221 targets. We have used mass spectrometry to identify and sequence a novel peptide from HLA-E following heat shock, ALALVRMLI, derived from the transmembrane domain of the human ATP-binding cassette protein, multidrug resistance-associated protein, MRP7. Pulsing 721.221 targets with synthetic MRP7 peptide results in strong inhibition of NK cell-mediated lysis that is reversible using anti-CD94 and anti-class I mAbs. This report is the first to identify a non-MHC leader inhibitory peptide bound to HLA-E and provides insight into the immunoregulatory role of HLA-E during cell stress.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ABCC10 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/ATP-Binding Cassette Transporters,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, B-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/HLA Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-E antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class I,
http://linkedlifedata.com/resource/pubmed/chemical/Immunologic Factors,
http://linkedlifedata.com/resource/pubmed/chemical/KLRC1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/KLRD1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins, C-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Multidrug Resistance-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/NK Cell Lectin-Like Receptor...,
http://linkedlifedata.com/resource/pubmed/chemical/NK Cell Lectin-Like Receptor...,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Natural Killer Cell
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
175
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1383-7
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:16034073-ATP-Binding Cassette Transporters,
pubmed-meshheading:16034073-Antigens, CD,
pubmed-meshheading:16034073-Cell Line,
pubmed-meshheading:16034073-Cytotoxicity, Immunologic,
pubmed-meshheading:16034073-Cytotoxicity Tests, Immunologic,
pubmed-meshheading:16034073-Epitopes, B-Lymphocyte,
pubmed-meshheading:16034073-HLA Antigens,
pubmed-meshheading:16034073-Histocompatibility Antigens Class I,
pubmed-meshheading:16034073-Hot Temperature,
pubmed-meshheading:16034073-Humans,
pubmed-meshheading:16034073-Immunologic Factors,
pubmed-meshheading:16034073-Killer Cells, Natural,
pubmed-meshheading:16034073-Lectins, C-Type,
pubmed-meshheading:16034073-Multidrug Resistance-Associated Proteins,
pubmed-meshheading:16034073-NK Cell Lectin-Like Receptor Subfamily C,
pubmed-meshheading:16034073-NK Cell Lectin-Like Receptor Subfamily D,
pubmed-meshheading:16034073-Osmotic Pressure,
pubmed-meshheading:16034073-Peptide Fragments,
pubmed-meshheading:16034073-Protein Binding,
pubmed-meshheading:16034073-Receptors, Immunologic,
pubmed-meshheading:16034073-Receptors, Natural Killer Cell
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pubmed:year |
2005
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pubmed:articleTitle |
Cutting edge: HLA-E binds a peptide derived from the ATP-binding cassette transporter multidrug resistance-associated protein 7 and inhibits NK cell-mediated lysis.
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pubmed:affiliation |
Department of Molecular Microbiology and Immunology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD 21205, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, N.I.H., Extramural
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