| pubmed-article:16033530 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16033530 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:16033530 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:16033530 | lifeskim:mentions | umls-concept:C0039195 | lld:lifeskim |
| pubmed-article:16033530 | lifeskim:mentions | umls-concept:C0003250 | lld:lifeskim |
| pubmed-article:16033530 | lifeskim:mentions | umls-concept:C0003241 | lld:lifeskim |
| pubmed-article:16033530 | lifeskim:mentions | umls-concept:C1420728 | lld:lifeskim |
| pubmed-article:16033530 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:16033530 | lifeskim:mentions | umls-concept:C0332206 | lld:lifeskim |
| pubmed-article:16033530 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:16033530 | pubmed:dateCreated | 2005-7-21 | lld:pubmed |
| pubmed-article:16033530 | pubmed:abstractText | Thy-1 (CD90) expressed by mouse T cells is known to have signal transducing properties, but the ability of Thy-1 to enhance cytotoxic T lymphocyte (CTL) development is not well understood. Here we show that stimulation of mouse T cells with monoclonal antibodies (mAb) to CD3, CD28 and Thy-1 (clone G7), which were coimmobilized on polystyrene microbeads, resulted in a greater proliferative response than stimulation with only anti-CD3 and anti-CD28 mAb, indicating that Thy-1 cross-linking enhanced T cell receptor/CD28-driven T cell activation. Consistent with this finding, Thy-1 blockade with a soluble nonactivating anti-Thy-1 mAb (clone 30-H12) inhibited anti-CD3-induced proliferation of CD4+ and CD8+ T cells, and the induction of cytotoxic effector cells in a dose-dependent fashion. Interleukin-2 synthesis and CD25 expression were also impaired by Thy-1 blockade. The inhibitory effect involved a defect at or before the level of protein kinase C activation because the addition of phorbol ester ablated the anti-Thy-1-mediated inhibition of anti-CD3-induced T cell activation. The CTL that were induced in the presence of blocking anti-Thy-1 mAb adhered to target cells but showed reduced expression of granzyme B and perforin. In contrast, Fas ligand expression and function was not affected by Thy-1 blockade. We conclude that Thy-1 signalling promotes the in vitro generation of CTL that kill in a granule-dependent fashion. | lld:pubmed |
| pubmed-article:16033530 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16033530 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16033530 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16033530 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16033530 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16033530 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16033530 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16033530 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16033530 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16033530 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16033530 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16033530 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16033530 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16033530 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16033530 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16033530 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16033530 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16033530 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16033530 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:16033530 | pubmed:issn | 0818-9641 | lld:pubmed |
| pubmed-article:16033530 | pubmed:author | pubmed-author:HoskinDavid... | lld:pubmed |
| pubmed-article:16033530 | pubmed:author | pubmed-author:HaeryfarS M... | lld:pubmed |
| pubmed-article:16033530 | pubmed:author | pubmed-author:ConradDavid... | lld:pubmed |
| pubmed-article:16033530 | pubmed:author | pubmed-author:MusgraveBruce... | lld:pubmed |
| pubmed-article:16033530 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16033530 | pubmed:volume | 83 | lld:pubmed |
| pubmed-article:16033530 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16033530 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16033530 | pubmed:pagination | 352-63 | lld:pubmed |
| pubmed-article:16033530 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:16033530 | pubmed:meshHeading | pubmed-meshheading:16033530... | lld:pubmed |
| pubmed-article:16033530 | pubmed:meshHeading | pubmed-meshheading:16033530... | lld:pubmed |
| pubmed-article:16033530 | pubmed:meshHeading | pubmed-meshheading:16033530... | lld:pubmed |
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| pubmed-article:16033530 | pubmed:meshHeading | pubmed-meshheading:16033530... | lld:pubmed |
| pubmed-article:16033530 | pubmed:meshHeading | pubmed-meshheading:16033530... | lld:pubmed |
| pubmed-article:16033530 | pubmed:meshHeading | pubmed-meshheading:16033530... | lld:pubmed |
| pubmed-article:16033530 | pubmed:meshHeading | pubmed-meshheading:16033530... | lld:pubmed |
| pubmed-article:16033530 | pubmed:meshHeading | pubmed-meshheading:16033530... | lld:pubmed |
| pubmed-article:16033530 | pubmed:meshHeading | pubmed-meshheading:16033530... | lld:pubmed |
| pubmed-article:16033530 | pubmed:meshHeading | pubmed-meshheading:16033530... | lld:pubmed |
| pubmed-article:16033530 | pubmed:meshHeading | pubmed-meshheading:16033530... | lld:pubmed |
| pubmed-article:16033530 | pubmed:meshHeading | pubmed-meshheading:16033530... | lld:pubmed |
| pubmed-article:16033530 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:16033530 | pubmed:articleTitle | Antibody blockade of Thy-1 (CD90) impairs mouse cytotoxic T lymphocyte induction by anti-CD3 monoclonal antibody. | lld:pubmed |
| pubmed-article:16033530 | pubmed:affiliation | Department of Microbiology and Immunology, Faculty of Medicine, Dalhousie University, Halifax, Canada. | lld:pubmed |
| pubmed-article:16033530 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16033530 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:21838 | entrezgene:pubmed | pubmed-article:16033530 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:16033530 | lld:entrezgene |
