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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2005-7-21
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pubmed:abstractText |
Thy-1 (CD90) expressed by mouse T cells is known to have signal transducing properties, but the ability of Thy-1 to enhance cytotoxic T lymphocyte (CTL) development is not well understood. Here we show that stimulation of mouse T cells with monoclonal antibodies (mAb) to CD3, CD28 and Thy-1 (clone G7), which were coimmobilized on polystyrene microbeads, resulted in a greater proliferative response than stimulation with only anti-CD3 and anti-CD28 mAb, indicating that Thy-1 cross-linking enhanced T cell receptor/CD28-driven T cell activation. Consistent with this finding, Thy-1 blockade with a soluble nonactivating anti-Thy-1 mAb (clone 30-H12) inhibited anti-CD3-induced proliferation of CD4+ and CD8+ T cells, and the induction of cytotoxic effector cells in a dose-dependent fashion. Interleukin-2 synthesis and CD25 expression were also impaired by Thy-1 blockade. The inhibitory effect involved a defect at or before the level of protein kinase C activation because the addition of phorbol ester ablated the anti-Thy-1-mediated inhibition of anti-CD3-induced T cell activation. The CTL that were induced in the presence of blocking anti-Thy-1 mAb adhered to target cells but showed reduced expression of granzyme B and perforin. In contrast, Fas ligand expression and function was not affected by Thy-1 blockade. We conclude that Thy-1 signalling promotes the in vitro generation of CTL that kill in a granule-dependent fashion.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Thy-1,
http://linkedlifedata.com/resource/pubmed/chemical/Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Granzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Gzmb protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Pore Forming Cytotoxic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/perforin, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0818-9641
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
352-63
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:16033530-Animals,
pubmed-meshheading:16033530-Antibodies, Monoclonal,
pubmed-meshheading:16033530-Antigens, CD3,
pubmed-meshheading:16033530-Antigens, Differentiation,
pubmed-meshheading:16033530-Antigens, Thy-1,
pubmed-meshheading:16033530-Cell Proliferation,
pubmed-meshheading:16033530-Endopeptidases,
pubmed-meshheading:16033530-Gene Expression Regulation,
pubmed-meshheading:16033530-Granzymes,
pubmed-meshheading:16033530-Interleukin-2,
pubmed-meshheading:16033530-Membrane Proteins,
pubmed-meshheading:16033530-Mice,
pubmed-meshheading:16033530-Mice, Inbred C57BL,
pubmed-meshheading:16033530-Pore Forming Cytotoxic Proteins,
pubmed-meshheading:16033530-Protein Kinase C,
pubmed-meshheading:16033530-RNA, Messenger,
pubmed-meshheading:16033530-Receptors, Interleukin-2,
pubmed-meshheading:16033530-T-Lymphocytes, Cytotoxic
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pubmed:year |
2005
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pubmed:articleTitle |
Antibody blockade of Thy-1 (CD90) impairs mouse cytotoxic T lymphocyte induction by anti-CD3 monoclonal antibody.
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pubmed:affiliation |
Department of Microbiology and Immunology, Faculty of Medicine, Dalhousie University, Halifax, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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