Source:http://linkedlifedata.com/resource/pubmed/id/16033428
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2005-7-21
|
| pubmed:databankReference | |
| pubmed:abstractText |
In the silkworm, Bombyx mori (female, ZW; male, ZZ), femaleness is determined by the presence of a single W chromosome, irrespective of the number of autosomes or Z chromosomes. The W chromosome is devoid of functional genes, except the putative female-determining gene (Fem). However, there are strains in which chromosomal fragments containing autosomal markers have been translocated on to W. In this study, we analysed the W chromosomal regions of the Zebra-W strain (T(W;3)Ze chromosome) and the Black-egg-W strain (T(W;10)+(w-2) chromosome) at the molecular level. Initially, we undertook a project to identify W-specific RAPD markers, in addition to the three already established W-specific RAPD markers (W-Kabuki, W-Samurai and W-Kamikaze). Following the screening of 3648 arbitrary 10-mer primers, we obtained nine W-specific RAPD marker sequences (W-Bonsai, W-Mikan, W-Musashi, W-Rikishi, W-Sakura, W-Sasuke, W-Yukemuri-L, W-Yukemuri-S and BMC1-Kabuki), almost all of which contained the border regions of retrotransposons, namely portions of nested retrotransposons. We confirmed the presence of eleven out of twelve W-specific RAPD markers in the normal W chromosomes of twenty-five silkworm strains maintained in Japan. These results indicate that the W chromosomes of the strains in Japan are almost identical in type. The Zebra-W strain (T(W;3)Ze chromosome) lacked the W-Samurai and W-Mikan RAPD markers and the Black-egg-W strain (T(W;10)+(w-2) chromosome) lacked the W-Mikan RAPD marker. These results strongly indicate that the regions containing the W-Samurai and W-Mikan RAPD markers or the W-Mikan RAPD marker were deleted in the T(W;3)Ze and T(W;10)+(w-2) chromosomes, respectively, due to reciprocal translocation between the W chromosome and the autosome. This deletion apparently does not affect the expression of Fem; therefore, this deleted region of the W chromosome does not contain the putative Fem gene.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0962-1075
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| pubmed:author |
pubmed-author:AbeHH,
pubmed-author:AjimuraMM,
pubmed-author:BannoYY,
pubmed-author:FujiiTT,
pubmed-author:IharaJJ,
pubmed-author:MithRR,
pubmed-author:OhbayashiFF,
pubmed-author:OshikiTT,
pubmed-author:SaharaKK,
pubmed-author:SekiMM,
pubmed-author:ShimadaTT,
pubmed-author:SuzukiM GMG,
pubmed-author:TakahashiMM,
pubmed-author:TanakaNN,
pubmed-author:YamashitaJJ,
pubmed-author:YasukochiYY,
pubmed-author:YokoyamaTT,
pubmed-author:YoshidoAA
|
| pubmed:issnType |
Print
|
| pubmed:volume |
14
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
339-52
|
| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16033428-Animals,
pubmed-meshheading:16033428-Base Sequence,
pubmed-meshheading:16033428-Bombyx,
pubmed-meshheading:16033428-Chromosome Deletion,
pubmed-meshheading:16033428-Chromosomes, Artificial, Bacterial,
pubmed-meshheading:16033428-DNA,
pubmed-meshheading:16033428-Female,
pubmed-meshheading:16033428-Gene Library,
pubmed-meshheading:16033428-Genetic Markers,
pubmed-meshheading:16033428-In Situ Hybridization, Fluorescence,
pubmed-meshheading:16033428-Male,
pubmed-meshheading:16033428-Molecular Sequence Data,
pubmed-meshheading:16033428-Random Amplified Polymorphic DNA Technique,
pubmed-meshheading:16033428-Retroelements,
pubmed-meshheading:16033428-Sex Chromosome Aberrations,
pubmed-meshheading:16033428-Sex Chromosomes,
pubmed-meshheading:16033428-Translocation, Genetic
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| pubmed:year |
2005
|
| pubmed:articleTitle |
Partial deletions of the W chromosome due to reciprocal translocation in the silkworm Bombyx mori.
|
| pubmed:affiliation |
Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Japan. wfem@cc.tuat.ac.jp
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|