|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
30
|
|
pubmed:dateCreated |
2005-7-27
|
|
pubmed:abstractText |
Proinsulin is a key autoantigen in type 1 diabetes. Evidence in the mouse has underscored the importance of the insulin B chain region in autoimmunity to pancreatic beta cells. In man, a majority of proteasome cleavage sites are predicted by proteasome cleavage algorithms within this region. To study CD8+ T cell responses to the insulin B chain and adjacent C peptide, we selected 8- to 11-mer peptides according to proteasome cleavage patterns obtained by digestion of two peptides covering proinsulin residues 28 to 64. We studied their binding to purified HLA class I molecules and their recognition by T cells from diabetic patients. Peripheral blood mononuclear cells from 17 of 19 recent-onset and 12 of 13 long-standing type 1 diabetic patients produced IFN-gamma in response to proinsulin peptides as shown by using an ELISPOT assay. In most patients, the response was against several class I-restricted peptides. Nine peptides were recognized within the proinsulin region covering residues 34 to 61. Four yielded a high frequency of recognition in HLA-A1 and -B8 patients. Three peptides located in the proinsulin region 41-51 were shown to bind several HLA molecules and to be recognized in a high percentage of diabetic patients.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-10078544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-10226887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-10470079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-10570321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-10602880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-10637269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-10677521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-11136822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-11160133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-11342637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-11586956,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-11673498,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-12239297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-12639991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-12925730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-1385478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-14578281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-14617048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-14688305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-14745491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-15224091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-15224092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-1744803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-2204345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-2451692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-3159965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-3894793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-7537670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-7705402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-7722443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-7722468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-7913115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-8090207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-8227346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-8400618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-8408615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-8529129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-8570667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-8871671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-8962628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-9115575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-9153283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-9165225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-9405907,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16030147-9438206
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
pubmed-author:BoitardChristianC,
pubmed-author:BriandJean-PaulJP,
pubmed-author:Caillat-ZucmanSophieS,
pubmed-author:CamoinLucL,
pubmed-author:CarelJean-ClaudeJC,
pubmed-author:ChoppinJeannineJ,
pubmed-author:ConnanFrancineF,
pubmed-author:Dubois-LaforgueDanielleD,
pubmed-author:GaheryHanneH,
pubmed-author:GuilletJean-GérardJG,
pubmed-author:HaddoukSamyS,
pubmed-author:MullerSylvianeS,
pubmed-author:TomaAndréaA
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
26
|
|
pubmed:volume |
102
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
10581-6
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:16030147-Adult,
pubmed-meshheading:16030147-Aged,
pubmed-meshheading:16030147-CD8-Positive T-Lymphocytes,
pubmed-meshheading:16030147-Diabetes Mellitus, Type 1,
pubmed-meshheading:16030147-Female,
pubmed-meshheading:16030147-Histocompatibility Antigens Class I,
pubmed-meshheading:16030147-Humans,
pubmed-meshheading:16030147-Immunoenzyme Techniques,
pubmed-meshheading:16030147-Interferon-gamma,
pubmed-meshheading:16030147-Male,
pubmed-meshheading:16030147-Mass Spectrometry,
pubmed-meshheading:16030147-Middle Aged,
pubmed-meshheading:16030147-Proinsulin
|
|
pubmed:year |
2005
|
|
pubmed:articleTitle |
Recognition of a subregion of human proinsulin by class I-restricted T cells in type 1 diabetic patients.
|
