Source:http://linkedlifedata.com/resource/pubmed/id/16030067
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2005-11-10
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| pubmed:abstractText |
Chronic arthritis is a catabolic state associated with an inhibition of the IGF system and a decrease in body weight. Cachexia and muscular wasting is secondary to protein degradation by the ubiquitin-proteasome pathway. The aim of this work was to analyze the effect of adjuvant-induced arthritis on the muscle-specific ubiquitin ligases muscle ring finger 1 (MuRF1) and muscle atrophy F-box (MAFbx) as well as on IGF-I and IGF-binding protein-5 (IGFBP-5) gene expression in the skeletal muscle. We also studied whether the synthetic ghrelin receptor agonist, growth hormone releasing peptide-2 (GHRP-2), was able to prevent arthritis-induced changes in the skeletal muscle. Arthritis induced an increase in MuRF1, MAFbx (P < 0.01), and tumor necrosis factor (TNF)-alpha mRNA (P < 0.05) in the skeletal muscle. Arthritis decreased the serum IGF-I and its gene expression in the liver (P < 0.01), whereas it increased IGF-I and IGFBP-5 gene expression in the skeletal muscle (P < 0.01). Administration of GHRP-2 for 8 days prevented the arthritis-induced increase in muscular MuRF1, MAFbx, and TNF-alpha gene expression. GHRP-2 treatment increased the serum concentrations of IGF-I and the IGF-I mRNA in the liver and in the cardiac muscle and decreased muscular IGFBP-5 mRNA both in control and in arthritic rats (P < 0.05). GHRP-2 treatment increased muscular IGF-I mRNA in control rats (P < 0.01), but it did not modify the muscular IGF-I gene expression in arthritic rats. These data indicate that arthritis induces an increase in the activity of the ubiquitin-proteasome proteolytic pathway that is prevented by GHRP-2 administration. The parallel changes in muscular IGFBP-5 and TNF-alpha gene expression with the ubiquitin ligases suggest that they can participate in skeletal muscle alterations during chronic arthritis.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Fbxo32 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor I,
http://linkedlifedata.com/resource/pubmed/chemical/Muscle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, G-Protein-Coupled,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Ghrelin,
http://linkedlifedata.com/resource/pubmed/chemical/Rnf28 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/SKP Cullin F-Box Protein Ligases,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin-Protein Ligases,
http://linkedlifedata.com/resource/pubmed/chemical/growth hormone-releasing peptide-2
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| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
0193-1849
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
289
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
E1007-14
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16030067-Animals,
pubmed-meshheading:16030067-Arthritis, Experimental,
pubmed-meshheading:16030067-Gene Expression,
pubmed-meshheading:16030067-Insulin-Like Growth Factor Binding Protein 5,
pubmed-meshheading:16030067-Insulin-Like Growth Factor I,
pubmed-meshheading:16030067-Liver,
pubmed-meshheading:16030067-Male,
pubmed-meshheading:16030067-Muscle, Skeletal,
pubmed-meshheading:16030067-Muscle Proteins,
pubmed-meshheading:16030067-Oligopeptides,
pubmed-meshheading:16030067-RNA, Messenger,
pubmed-meshheading:16030067-Rats,
pubmed-meshheading:16030067-Rats, Wistar,
pubmed-meshheading:16030067-Receptors, G-Protein-Coupled,
pubmed-meshheading:16030067-Receptors, Ghrelin,
pubmed-meshheading:16030067-SKP Cullin F-Box Protein Ligases,
pubmed-meshheading:16030067-Ubiquitin-Protein Ligases
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| pubmed:year |
2005
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| pubmed:articleTitle |
Ghrelin receptor agonist GHRP-2 prevents arthritis-induced increase in E3 ubiquitin-ligating enzymes MuRF1 and MAFbx gene expression in skeletal muscle.
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| pubmed:affiliation |
Dept Fisiología, Facultad de Medicina, Universidad Complutense, 28040 Madrid, Spain.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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