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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
29
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pubmed:dateCreated |
2005-7-20
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pubmed:abstractText |
An artificial polypeptide scaffold composed of surface anchor and protein capture domains was designed and expressed in vivo. By using a mutant E. coli phenylalanyl-tRNA synthetase, the photoreactive amino acid para-azidophenylalanine was incorporated into the surface anchor domain. Octyltrichlorosilane-treated surfaces were functionalized with this polypeptide by spin coating and photocrosslinking. The resulting protein films were shown to immobilize recombinant proteins through association of coiled coil heterodimer.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0002-7863
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
127
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10136-7
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pubmed:dateRevised |
2008-1-17
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pubmed:meshHeading |
pubmed-meshheading:16028902-Amino Acid Sequence,
pubmed-meshheading:16028902-Escherichia coli,
pubmed-meshheading:16028902-Glutathione Transferase,
pubmed-meshheading:16028902-Green Fluorescent Proteins,
pubmed-meshheading:16028902-Leucine Zippers,
pubmed-meshheading:16028902-Molecular Sequence Data,
pubmed-meshheading:16028902-Peptides,
pubmed-meshheading:16028902-Phenylalanine-tRNA Ligase,
pubmed-meshheading:16028902-Protein Array Analysis,
pubmed-meshheading:16028902-Recombinant Proteins,
pubmed-meshheading:16028902-Silanes
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pubmed:year |
2005
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pubmed:articleTitle |
Artificial polypeptide scaffold for protein immobilization.
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pubmed:affiliation |
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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