Linked Life Data

16028121

Source:http://linkedlifedata.com/resource/pubmed/id/16028121

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2005-7-19
pubmed:databankReference
pubmed:abstractText
ZmOCL1 is the founding member of the ZmOCL (Outer Cell Layer) family encoding putative transcription factors of the HD-ZIP IV class. It is expressed in the L1 cell layer of the embryo and several other tissues of maize. After determination of the intron/exon structure a mutator insertion was isolated in the upstream region. No notable phenotypes and wildtype levels of ZmOCL1 transcript were observed in homozygous mutant plants. In contrast transgenic plants carrying a fusion of the repressor domain of the Drosophila Engrailed gene with the DNA binding and dimerisation domains of ZmOCL1 showed a transient reduction of embryo, endosperm and kernel size that was most obvious around 15 DAP. An inverse relationship was observed between the degree of size reduction and the expression level of the transcript. In reciprocal crosses the size reduction was only observed when the transgenic plants were used as females and no expression of male transmitted transgenes was detected. Smaller kernels resembled younger kernels of wild-type siblings indicating that interference with ZmOCL1 function leads to an overall slow-down of early kernel development. Based on marker gene analysis ZmOCL1 may act via a modification of gibberellin levels. Phylogenetic analyses based on the intron/exon structure and sequence similarities of ZmOCL1 and other HD-ZIP IV proteins from maize, rice and Arabidopsis helped to identify orthologues and suggested an evolution in the function of individual genes after the divergence of monocots and dicots.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0167-4412
pubmed:author
pubmed:issnType
Print
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
123-39
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16028121-Binding Sites, pubmed-meshheading:16028121-DNA, Plant, pubmed-meshheading:16028121-Drosophila Proteins, pubmed-meshheading:16028121-Exons, pubmed-meshheading:16028121-Gene Expression Regulation, Developmental, pubmed-meshheading:16028121-Gene Expression Regulation, Plant, pubmed-meshheading:16028121-Genes, Plant, pubmed-meshheading:16028121-Homeodomain Proteins, pubmed-meshheading:16028121-Introns, pubmed-meshheading:16028121-Membrane Proteins, pubmed-meshheading:16028121-Molecular Sequence Data, pubmed-meshheading:16028121-Mutagenesis, Insertional, pubmed-meshheading:16028121-Mutation, pubmed-meshheading:16028121-Phylogeny, pubmed-meshheading:16028121-Plant Proteins, pubmed-meshheading:16028121-Plants, Genetically Modified, pubmed-meshheading:16028121-Recombinant Fusion Proteins, pubmed-meshheading:16028121-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16028121-Seeds, pubmed-meshheading:16028121-Sequence Analysis, DNA, pubmed-meshheading:16028121-Transcription Factors, pubmed-meshheading:16028121-Zea mays
pubmed:year
2005
pubmed:articleTitle
Engrailed-ZmOCL1 fusions cause a transient reduction of kernel size in maize.
pubmed:affiliation
RDP, UMR879 INRA-CNRS-ENSL-UCBL, IFR128 BioSciences Lyon-Gerland, ENS-Lyon 46 Allée d'Italie, F-69364, Lyon Cedex 07, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't