Linked Life Data

16027891

Source:http://linkedlifedata.com/resource/pubmed/id/16027891

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2005-7-19
pubmed:abstractText
A monolayer of dissociated glial cells of human olfactory epithelium was cultured in Petri dishes and 12-well plates using a polylysine-laminin substrate. Primary cultures were subcultured after 10-15 days. The cell cultures were analyzed by phase contrast microscopy at all stages of culturing. A cytological study involved histological methods (trypan blue staining) and immunocytochemical visualization of GFAP, nestin, and low-affinity nerve growth factor receptors. At the final stage of culturing (5 passages) the monolayer cultures included 2 types of cells: GFAP- and p75-positive glial cells and nestin-positive fibroblasts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0007-4888
pubmed:author
pubmed:issnType
Print
pubmed:volume
139
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
510-3
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Culturing of specialized glial cells (olfactory ensheathing cells) of human olfactory epithelium.
pubmed:affiliation
Department of Biological Psychiatry, V. P. Serbskii State Research Center of Social and Forensic Medicine, Russia.
pubmed:publicationType
Journal Article