Linked Life Data

16025921

Source:http://linkedlifedata.com/resource/pubmed/id/16025921

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2005-7-19
pubmed:abstractText
The authors investigated the effects of inhalation of diesel exhaust (DE) on murine mycobacterial infection in vivo. Eight-week-old female BALB/c mice were exposed to DE (3 mg/m3 of diesel exhaust particles [DEPs]) for 1 month, 2 months, or 6 months (for 7 hours a day, 5 days a week). Control mice were housed in a clean room for the same periods. On the day following the last DE exposure, control mice and DE-exposed mice were aerially infected with Mycobacterium tuberculosis (1 x 10(6) colony-forming units (CFU), Kurono strain). At 7 weeks after mycobacterial infection, the authors examined the lung tissues for histopathological changes and performed reverse transcriptase-polymerase chain reaction (RT-PCR) to measure the messenger RNA (mRNA) expression of several proinflammatory cytokines and inducible nitric oxide synthase (iNOS). Then, the homogenates of lungs and spleens were cultured on 1% (v/v) Ogawa's egg slant medium, and after a 4-week incubation period at 37 degrees C, colonies on the medium were counted. After 1 month of DE exposure, the mycobacterial infection had slightly ameliorated. After 2 months of DE exposure, the expression levels of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, IL-12p40, interferon (IFN)-gamma, and iNOS mRNAs were slightly increased. However, after 6 months of DE exposure, the expression levels of IL-1beta , IL-12p40, IFN-gamma, and iNOS mRNAs were decreased, and the infection as measured by increased lung burden (CFU) actually increased. These results indicate that long-term DE exposure may increase pulmonary mycobacterial burden.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0190-2148
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
405-15
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16025921-Administration, Inhalation, pubmed-meshheading:16025921-Animals, pubmed-meshheading:16025921-Cytokines, pubmed-meshheading:16025921-Disease Susceptibility, pubmed-meshheading:16025921-Female, pubmed-meshheading:16025921-Lung, pubmed-meshheading:16025921-Mice, pubmed-meshheading:16025921-Mice, Inbred BALB C, pubmed-meshheading:16025921-Mycobacterium tuberculosis, pubmed-meshheading:16025921-Nitric Oxide Synthase, pubmed-meshheading:16025921-Nitric Oxide Synthase Type II, pubmed-meshheading:16025921-RNA, Messenger, pubmed-meshheading:16025921-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16025921-Spleen, pubmed-meshheading:16025921-Time Factors, pubmed-meshheading:16025921-Tuberculosis, Pulmonary, pubmed-meshheading:16025921-Vehicle Emissions
pubmed:year
2005
pubmed:articleTitle
The effects of inhalation of diesel exhaust on murine mycobacterial infection.
pubmed:affiliation
Fourth Department of Internal Medicine, Nippon Medical School, Tokyo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't