| pubmed-article:16025148 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16025148 | lifeskim:mentions | umls-concept:C0006826 | lld:lifeskim |
| pubmed-article:16025148 | lifeskim:mentions | umls-concept:C2004454 | lld:lifeskim |
| pubmed-article:16025148 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:16025148 | lifeskim:mentions | umls-concept:C0449851 | lld:lifeskim |
| pubmed-article:16025148 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:16025148 | lifeskim:mentions | umls-concept:C0439831 | lld:lifeskim |
| pubmed-article:16025148 | lifeskim:mentions | umls-concept:C0022130 | lld:lifeskim |
| pubmed-article:16025148 | pubmed:issue | 9 | lld:pubmed |
| pubmed-article:16025148 | pubmed:dateCreated | 2005-8-19 | lld:pubmed |
| pubmed-article:16025148 | pubmed:abstractText | Hypermethylation of CpG islands is a phenomenon commonly observed during the development and progression of human tumors. Detection of methylated-CpG islands in easily accessible biological materials such as serum has the potential to be useful for the early diagnosis of cancer. Most currently used methods for detecting methylated-CpG islands are based on sodium bisulfite conversion of genomic DNA, followed by PCR reactions. Here we describe a method, methylated-CpG island recovery assay (MIRA) that does not depend on the use of sodium bisulfite but has similar sensitivity and specificity as bisulfite-based approaches. Methyl-CpG-binding domain proteins, such as methyl-CpG-binding domain protein-2 (MBD2), have the capacity to bind specifically to methylated DNA sequences. In the MIRA procedure, sonicated genomic DNA isolated from cells or tissue is incubated with a matrix containing glutathione-S-transferase-MBD2b in the presence of methyl-CpG-binding domain protein 3-like-1, a binding partner of MBD2 that increases the affinity of MBD2 for methylated DNA. Specifically bound DNA is eluted from the matrix and gene-specific PCR reactions are performed to detect CpG island methylation. Methylation can be detected using 1 ng of DNA or 3000 cells. MIRA is a specific and sensitive, but not laborious, technique that can be clinically useful in the detection and diagnosis of any DNA methylation-associated disease, including cancer. | lld:pubmed |
| pubmed-article:16025148 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16025148 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16025148 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16025148 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16025148 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16025148 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16025148 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16025148 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:16025148 | pubmed:issn | 0023-6837 | lld:pubmed |
| pubmed-article:16025148 | pubmed:author | pubmed-author:PfeiferGerd... | lld:pubmed |
| pubmed-article:16025148 | pubmed:author | pubmed-author:RauchTiborT | lld:pubmed |
| pubmed-article:16025148 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16025148 | pubmed:volume | 85 | lld:pubmed |
| pubmed-article:16025148 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16025148 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16025148 | pubmed:pagination | 1172-80 | lld:pubmed |
| pubmed-article:16025148 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:16025148 | pubmed:meshHeading | pubmed-meshheading:16025148... | lld:pubmed |
| pubmed-article:16025148 | pubmed:meshHeading | pubmed-meshheading:16025148... | lld:pubmed |
| pubmed-article:16025148 | pubmed:meshHeading | pubmed-meshheading:16025148... | lld:pubmed |
| pubmed-article:16025148 | pubmed:meshHeading | pubmed-meshheading:16025148... | lld:pubmed |
| pubmed-article:16025148 | pubmed:meshHeading | pubmed-meshheading:16025148... | lld:pubmed |
| pubmed-article:16025148 | pubmed:meshHeading | pubmed-meshheading:16025148... | lld:pubmed |
| pubmed-article:16025148 | pubmed:meshHeading | pubmed-meshheading:16025148... | lld:pubmed |
| pubmed-article:16025148 | pubmed:meshHeading | pubmed-meshheading:16025148... | lld:pubmed |
| pubmed-article:16025148 | pubmed:meshHeading | pubmed-meshheading:16025148... | lld:pubmed |
| pubmed-article:16025148 | pubmed:meshHeading | pubmed-meshheading:16025148... | lld:pubmed |
| pubmed-article:16025148 | pubmed:meshHeading | pubmed-meshheading:16025148... | lld:pubmed |
| pubmed-article:16025148 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:16025148 | pubmed:articleTitle | Methylated-CpG island recovery assay: a new technique for the rapid detection of methylated-CpG islands in cancer. | lld:pubmed |
| pubmed-article:16025148 | pubmed:affiliation | Division of Biology, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA. | lld:pubmed |
| pubmed-article:16025148 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16025148 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:16025148 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16025148 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16025148 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16025148 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16025148 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16025148 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16025148 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16025148 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16025148 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16025148 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16025148 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16025148 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16025148 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16025148 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16025148 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16025148 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16025148 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16025148 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16025148 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16025148 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16025148 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:16025148 | lld:pubmed |
