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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2005-7-18
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pubmed:abstractText |
A novel organic-inorganic nanocomposite of methylene blue (MB) and silicon oxide was synthesized and characterized by TEM, FTIR, and UV-vis. The as-prepared material was able to transfer the electron of the MB to electrode and was different from other SiO2 spheres structurally. It can be used as mediator to construct a biosensor with horseradish peroxidase (HRP) coimmobilized in the gelatine matrix and cross-linked with formaldehyde. The resulting biosensor exhibited fast amperometric response and good stability to hydrogen peroxide (H2O2). The linear range for H2O2 determination was from 1 x 10(-5) to 1.2 x 10(-3) M, with a detection limit of 4 x 10(-6) M based on S/N = 3. Moreover, the lifetime is more than 3 months under dry conditions at 4 degrees C.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0956-5663
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
372-7
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pubmed:dateRevised |
2009-7-14
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pubmed:meshHeading |
pubmed-meshheading:16023965-Biosensing Techniques,
pubmed-meshheading:16023965-Coated Materials, Biocompatible,
pubmed-meshheading:16023965-Electrochemistry,
pubmed-meshheading:16023965-Enzymes, Immobilized,
pubmed-meshheading:16023965-Equipment Design,
pubmed-meshheading:16023965-Equipment Failure Analysis,
pubmed-meshheading:16023965-Horseradish Peroxidase,
pubmed-meshheading:16023965-Hydrogen Peroxide,
pubmed-meshheading:16023965-Methylene Blue,
pubmed-meshheading:16023965-Microelectrodes,
pubmed-meshheading:16023965-Nanostructures,
pubmed-meshheading:16023965-Silicon Dioxide,
pubmed-meshheading:16023965-Surface Properties
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pubmed:year |
2005
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pubmed:articleTitle |
Electrochemical study of a new methylene blue/silicon oxide nanocomposition mediator and its application for stable biosensor of hydrogen peroxide.
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pubmed:affiliation |
Key Lab of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210093, PR China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Evaluation Studies
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