Source:http://linkedlifedata.com/resource/pubmed/id/16022898
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
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| pubmed:dateCreated |
2005-7-18
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| pubmed:abstractText |
The causative agent of severe acute respiratory syndrome (SARS) has been identified as SARS-associated coronavirus (SARS-CoV), but the prophylactic treatment of SARS-CoV is still under investigation. We constructed a recombinant adenovirus containing a truncated N-terminal fragment of the SARS-CoV Spike (S) gene (from--45 to 1469, designated Ad-S(N)), which encoded a truncated S protein (490 amino-acid residues, a part of 672 amino-acid S1 subunit), and investigated whether this construct could induce effective immunity against SARS-CoV in Wistar rats. Rats were immunized either subcutaneously or intranasally with Ad-S(N) once a week for three consecutive weeks. Our results showed that all of the immunized animals generated humoral immunity against the SARS-CoV spike protein, and the sera of immunized rats showed strong capable of protecting from SARS-CoV infection in vitro. Histopathological examination did not find evident side effects in the immunized animals. These results indicate that an adenoviral-based vaccine carrying an N-terminal fragment of the Spike gene is able to elicit strong SARS-CoV-specific humoral immune responses in rats, and may be useful for the development of a protective vaccine against SARS-CoV infection.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Envelope Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/spike glycoprotein, coronavirus
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| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
0168-1702
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| pubmed:author |
pubmed-author:ChenHan-KuiHK,
pubmed-author:HuangBi-JunBJ,
pubmed-author:HuangJia-LingJL,
pubmed-author:HuangWenlinW,
pubmed-author:KeMiao-LaML,
pubmed-author:LiuRan-YiRY,
pubmed-author:TanWei-PingWP,
pubmed-author:WangJun-MeiJM,
pubmed-author:WuLi-ZhiLZ,
pubmed-author:ZengYi-XinYX,
pubmed-author:ZhangRu-HuaRH,
pubmed-author:ZhangYan-LingYL
|
| pubmed:issnType |
Print
|
| pubmed:volume |
112
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
24-31
|
| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16022898-Adenoviridae,
pubmed-meshheading:16022898-Animals,
pubmed-meshheading:16022898-Antibodies, Viral,
pubmed-meshheading:16022898-Antibody Specificity,
pubmed-meshheading:16022898-Cell Line,
pubmed-meshheading:16022898-Cercopithecus aethiops,
pubmed-meshheading:16022898-Genetic Vectors,
pubmed-meshheading:16022898-Humans,
pubmed-meshheading:16022898-Membrane Glycoproteins,
pubmed-meshheading:16022898-Neutralization Tests,
pubmed-meshheading:16022898-Rats,
pubmed-meshheading:16022898-Rats, Wistar,
pubmed-meshheading:16022898-SARS Virus,
pubmed-meshheading:16022898-Severe Acute Respiratory Syndrome,
pubmed-meshheading:16022898-Vaccination,
pubmed-meshheading:16022898-Vero Cells,
pubmed-meshheading:16022898-Viral Envelope Proteins,
pubmed-meshheading:16022898-Viral Vaccines
|
| pubmed:year |
2005
|
| pubmed:articleTitle |
Adenoviral expression of a truncated S1 subunit of SARS-CoV spike protein results in specific humoral immune responses against SARS-CoV in rats.
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| pubmed:affiliation |
State Key Laboratory for Oncology in South China, Cancer Center, Sun Yat-sen University, 651 Dong-feng Road East, Guangzhou 510060, China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|