Source:http://linkedlifedata.com/resource/pubmed/id/16019419
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2005-7-15
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| pubmed:abstractText |
Autologous chondrocyte transplantation (ACT) is a promising method to treat chondral and osteochondral defects. This study introduced a modified method for cell culture in ACT. Porcine chondrocytes were cultured for 3 weeks under low hydrostatic pressure at 250 Pa. The results showed that the dry weight of the cartilage-like membrane in the loading group was 3.0 times more than the control group (no loading) (p < 0.01), and cell numbers were significantly increased by 3.1 times (p < 0.01) after a 3-week culture. Compared with the fresh tissue sample, the mRNA expression of collagen II was not statistically different and the mRNA of aggrecan was only slightly decreased by 19%. These data suggest that the hydrostatic pressure at this level significantly increased the cell numbers and biosynthesis of cultured chondrocytes.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0300-8207
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
46
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
93-9
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| pubmed:meshHeading |
pubmed-meshheading:16019419-Animals,
pubmed-meshheading:16019419-Cell Culture Techniques,
pubmed-meshheading:16019419-Chondrocytes,
pubmed-meshheading:16019419-Collagen Type II,
pubmed-meshheading:16019419-Hydrostatic Pressure,
pubmed-meshheading:16019419-RNA, Messenger,
pubmed-meshheading:16019419-Swine,
pubmed-meshheading:16019419-Transplantation, Autologous
|
| pubmed:year |
2005
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| pubmed:articleTitle |
A modified cell culture method for autologous chondrocyte transplantation.
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| pubmed:affiliation |
Department of Orthopaedic Surgery, China Medical University Hospital, Taichung, Taiwan.
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| pubmed:publicationType |
Journal Article
|