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rdf:type |
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lifeskim:mentions |
umls-concept:C0010097,
umls-concept:C0013081,
umls-concept:C0019564,
umls-concept:C0022116,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0061467,
umls-concept:C0085140,
umls-concept:C0162512,
umls-concept:C0205374,
umls-concept:C0206529,
umls-concept:C0242910,
umls-concept:C1257890,
umls-concept:C1420120,
umls-concept:C1709059,
umls-concept:C2348867
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pubmed:issue |
5
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pubmed:dateCreated |
2005-8-30
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pubmed:abstractText |
Our goals were to identify biochemical markers for transient global ischemia-induced delayed neuronal death and test possible drug therapies against this neuronal damage. Four-vessel occlusion (4-VO) for 20 min was used as a rat model. The temporal expression profiles of three glutamate transporters (GLT-1, GLAST and EAAC1) were evaluated in the CA1 region of the hippocampus and the striatum. The protein levels of the GLT-1 were significantly down-regulated between 3 and 6 h after ischemia-reperfusion in the CA1 region and striatum, returned to the control (2-VO) levels 24 h after reperfusion and remained unchanged for up to 7 days. The levels of GLAST in the CA1 region and striatum, and EAAC1 in the CA1 region did not change after ischemia from 1 h to 7 days. Pretreatment with group III metabotropic glutamate receptor antagonist s-alpha-MCPA (20 microg/5 microl) 30 min prior to 4-VO significantly restored the GLT-1 levels in the CA1 region caused by global ischemia at both 3 and 6 h after reperfusion. The loss of pyramidal neurons in the CA1 region due to ischemia-reperfusion could also be prevented by intraventricular pretreatment with s-alpha-MCPA. The current findings pinpoint the significance of GLT-1 during ischemia/reperfusion and suggest a potential application of group III metabotropic glutamate receptor antagonist against ischemic/hypoxic neuronal damage.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-Methyl-4-chlorophenoxyacetic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Transport System X-AG,
http://linkedlifedata.com/resource/pubmed/chemical/Excitatory Amino Acid Transporter 1,
http://linkedlifedata.com/resource/pubmed/chemical/Excitatory Amino Acid Transporter 2,
http://linkedlifedata.com/resource/pubmed/chemical/Excitatory Amino Acid Transporter 3,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamate Plasma Membrane...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Metabotropic Glutamate,
http://linkedlifedata.com/resource/pubmed/chemical/Slc1a1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Slc1a3 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Symporters
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0028-3908
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
49
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
703-14
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16019039-2-Methyl-4-chlorophenoxyacetic Acid,
pubmed-meshheading:16019039-Amino Acid Transport System X-AG,
pubmed-meshheading:16019039-Animals,
pubmed-meshheading:16019039-Blotting, Western,
pubmed-meshheading:16019039-Down-Regulation,
pubmed-meshheading:16019039-Excitatory Amino Acid Transporter 1,
pubmed-meshheading:16019039-Excitatory Amino Acid Transporter 2,
pubmed-meshheading:16019039-Excitatory Amino Acid Transporter 3,
pubmed-meshheading:16019039-Glutamate Plasma Membrane Transport Proteins,
pubmed-meshheading:16019039-Hippocampus,
pubmed-meshheading:16019039-Injections, Intraventricular,
pubmed-meshheading:16019039-Ischemic Attack, Transient,
pubmed-meshheading:16019039-Male,
pubmed-meshheading:16019039-Neostriatum,
pubmed-meshheading:16019039-Pyramidal Cells,
pubmed-meshheading:16019039-Rats,
pubmed-meshheading:16019039-Rats, Sprague-Dawley,
pubmed-meshheading:16019039-Receptors, Metabotropic Glutamate,
pubmed-meshheading:16019039-Reperfusion Injury,
pubmed-meshheading:16019039-Symporters
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pubmed:year |
2005
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pubmed:articleTitle |
Down-regulation of the glial glutamate transporter GLT-1 in rat hippocampus and striatum and its modulation by a group III metabotropic glutamate receptor antagonist following transient global forebrain ischemia.
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pubmed:affiliation |
Department of Pharmacology, Chang-Gung University, 259 Wen-Hwa 1st Road, Tao-Yuan, Kwei-Shan, Taiwan, ROC. jinchen@mail.cgu.edu.tw
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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