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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1992-7-14
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pubmed:databankReference |
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pubmed:abstractText |
We report the identification of the product of the Plasmodium falciparum Pf11-1 gene and demonstrate that it is a gametocyte-specific protein that has a potential role in the rupture of the host erythrocyte and emergence of the gametes (gametogenesis). The Pf11-1 gene is a large locus (30 kb) whose sequence predicts a glutamic acid-rich polypeptide. Our identification of the Pf11-1 gene product as gametocyte specific was greatly facilitated by the isolation of a mutant parasite clone in which greater than 90% of the Pf11-1 gene was deleted. Molecular analysis of the mutant locus suggests that the underlying genetic mechanism is chromosome breakage and subsequent healing by the addition of telomere repeats. PCR-based analysis showed that similar DNA rearrangements occur commonly in small subpopulations of most laboratory strains, suggesting that the Pf11-1 locus represents a fragile chromosome region. Northern blot analysis demonstrates that a large Pf11-1 gene-specific transcript (much greater than 10 kb) is present in gametocytes but not in asexual blood stage parasites. The Pf11-1 protein was localized by electron microscopy to granules in the cytoplasm of gametocytes adjacent to the membrane of the parasitophorous vacuole. Following in vitro stimulation of gametogenesis, the Pf11-1 protein was found in the membrane of lysed erythrocytes, suggesting a role for Pf11-1 in erythrocyte rupture within the mosquito gut.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1600948-1544579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1600948-1707506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1600948-2225076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1600948-2270101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1600948-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1600948-2448875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1600948-2645212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1600948-2666152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1600948-2677678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1600948-2805070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1600948-2841111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1600948-2893257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1600948-3049911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1600948-3056622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1600948-3285206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1600948-3299700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1600948-3510452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1600948-3538420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1600948-3889657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1600948-3996185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1600948-6090935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1600948-6373014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1600948-6382613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1600948-781840
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:geneSymbol |
Pf11-1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2293-301
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1600948-Animals,
pubmed-meshheading:1600948-Antibodies, Monoclonal,
pubmed-meshheading:1600948-Base Sequence,
pubmed-meshheading:1600948-Chromosome Aberrations,
pubmed-meshheading:1600948-Chromosome Deletion,
pubmed-meshheading:1600948-Chromosome Disorders,
pubmed-meshheading:1600948-Chromosome Mapping,
pubmed-meshheading:1600948-Chromosomes,
pubmed-meshheading:1600948-Cloning, Molecular,
pubmed-meshheading:1600948-DNA,
pubmed-meshheading:1600948-DNA Probes,
pubmed-meshheading:1600948-Humans,
pubmed-meshheading:1600948-Malaria, Falciparum,
pubmed-meshheading:1600948-Microscopy, Immunoelectron,
pubmed-meshheading:1600948-Molecular Sequence Data,
pubmed-meshheading:1600948-Mutation,
pubmed-meshheading:1600948-Plasmodium falciparum,
pubmed-meshheading:1600948-Polymerase Chain Reaction,
pubmed-meshheading:1600948-Reproduction
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pubmed:year |
1992
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pubmed:articleTitle |
Gene inactivation of Pf11-1 of Plasmodium falciparum by chromosome breakage and healing: identification of a gametocyte-specific protein with a potential role in gametogenesis.
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pubmed:affiliation |
Unité de Parasitologie Expérimentale, CNRS URA 361, Institut Pasteur, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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