Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
41
pubmed:dateCreated
2005-9-15
pubmed:abstractText
Genome-wide expression profiling revealed overexpression of the gene encoding frizzled homologue 10 (FZD 10), a cell-surface receptor for molecules in the Wnt pathway, as a potential contributor to synovial sarcomas (SS). Northern blotting and immunohistochemical staining confirmed that expression levels of FZD 10 were very high in nearly all SS tumors and cell lines examined but absent in most normal organs or in some cancers arising in other tissues. Treatment of human SS cells with small-interfering RNA (siRNA) to FZD 10 decreased the amount of its product and suppressed growth of SS cells. Moreover, a polyclonal antibody specifically recognizing the extracellular domain (ECD) of FZD 10 was markedly effective in mediating ADCC against FZD 10-overexpressing synovial sarcoma cells in vitro. Injection of the antibody into SS xenografts in nude mice attenuated tumor growth, and TUNEL assays revealed clusters of apoptotic cells in antibody-treated xenografts. Taken together, these findings suggest that a humanized antibody against FZD 10 might be a promising treatment for patients with tumors that overexpress FZD 10; minimal or no adverse reactions would be expected because FZD 10 protein is not abundant in vital organs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0950-9232
pubmed:author
pubmed:copyrightInfo
Oncogene (2005) 24, 6201-6212.
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6201-12
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16007199-Amino Acid Sequence, pubmed-meshheading:16007199-Animals, pubmed-meshheading:16007199-Antibodies, pubmed-meshheading:16007199-Biopolymers, pubmed-meshheading:16007199-Cell Line, Tumor, pubmed-meshheading:16007199-DNA, pubmed-meshheading:16007199-Epitopes, pubmed-meshheading:16007199-Frizzled Receptors, pubmed-meshheading:16007199-Humans, pubmed-meshheading:16007199-Immunohistochemistry, pubmed-meshheading:16007199-Mice, pubmed-meshheading:16007199-Mice, Nude, pubmed-meshheading:16007199-Molecular Sequence Data, pubmed-meshheading:16007199-RNA, Messenger, pubmed-meshheading:16007199-RNA, Small Interfering, pubmed-meshheading:16007199-Receptors, Cell Surface, pubmed-meshheading:16007199-Receptors, G-Protein-Coupled, pubmed-meshheading:16007199-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16007199-Sarcoma, Synovial, pubmed-meshheading:16007199-Sequence Homology, Amino Acid
pubmed:year
2005
pubmed:articleTitle
Therapeutic potential of antibodies against FZD 10, a cell-surface protein, for synovial sarcomas.
pubmed:affiliation
Laboratory of Molecular Medicine, Human Genome Center, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't