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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2005-8-10
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pubmed:abstractText |
2-D peptide mapping is a novel technique for the relative quantification of membrane proteins (Scheurer S. et al., Proteomics 2005, in press). Using closely related metastatic and nonmetastatic teratocarcinoma cell lines as a model system, we have performed a comparative analysis of different biotinylation reagents, tryptic digestion procedures, and mass spectrometric techniques, with the aim to increase the number of proteins identified by 2-D peptide mapping. Our experience indicates that the LC-MALDI TOF/TOF technique is superior to LC-ESI MS/MS in terms of the number of proteins identified and confidence in protein identification. Furthermore, the best results were obtained by tryptic digestion of proteins eluted from a streptavidin column using a cleavable biotin derivative.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1615-9853
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3035-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16003826-Animals,
pubmed-meshheading:16003826-Biotin,
pubmed-meshheading:16003826-Biotinylation,
pubmed-meshheading:16003826-Cell Membrane,
pubmed-meshheading:16003826-Databases, Protein,
pubmed-meshheading:16003826-Humans,
pubmed-meshheading:16003826-Mass Spectrometry,
pubmed-meshheading:16003826-Membrane Proteins,
pubmed-meshheading:16003826-Models, Biological,
pubmed-meshheading:16003826-Models, Chemical,
pubmed-meshheading:16003826-Molecular Probes,
pubmed-meshheading:16003826-Peptide Mapping,
pubmed-meshheading:16003826-Peptides,
pubmed-meshheading:16003826-Proteins,
pubmed-meshheading:16003826-Proteomics,
pubmed-meshheading:16003826-Spectrometry, Mass, Electrospray Ionization,
pubmed-meshheading:16003826-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:16003826-Streptavidin,
pubmed-meshheading:16003826-Trypsin
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pubmed:year |
2005
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pubmed:articleTitle |
A comparison of different biotinylation reagents, tryptic digestion procedures, and mass spectrometric techniques for 2-D peptide mapping of membrane proteins.
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pubmed:affiliation |
Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology-Zurich, Wolfgang-Pauli-Str. 10, CH-8093 Zurich, Switzerland.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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