Source:http://linkedlifedata.com/resource/pubmed/id/16001266
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
2005-11-28
|
pubmed:abstractText |
A pro-inflammatory cytokine, interleukin 18 (IL-18), induces intracellular expression of IL-1 and the release of IL-6. IL-1 and IL-6 has been detected in anterior pituitary cells, suggesting that IL-18 is produced in anterior pituitary cells and may serve to aid immuno-endocrine regulation. In the present study, we addressed this hypothesis by investigating the intracellular localization of IL-18 and its receptor in bovine anterior pituitary gland. IL-18 mRNA and its protein were detected in the anterior pituitary gland by RT-PCR and Western blotting. In situ hybridization showed that IL-18 mRNA was expressed in the anterior pituitary cells. Immunohistochemistry of IL-18 and specific hormones revealed the presence of IL-18 in somatotrophs. Furthermore, the expression of GH mRNA in IL-18 immunoreactive cells was confirmed by immuno-laser microdissection. These results first demonstrated that somatotrophs produced IL-18. Subsequently, the distribution of the IL-18 receptor alpha (IL-18Ralpha) was investigated in order to understand IL-18 signaling among the anterior pituitary cells. Bovine IL-18Ralpha cDNA was partially sequenced and detected in the anterior pituitary gland by RT-PCR. Immunohistochemistry of IL-18Ralpha, IL-18 and GH showed that IL-18Ralpha was co-localized in IL-18 immunoreactive cells or somatotrophs. These data suggest that IL-18 acts on somatotrophs as an immuno-endocrine mediator through the autocrine pathway.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-18,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-18
|
pubmed:status |
MEDLINE
|
pubmed:month |
Dec
|
pubmed:issn |
0302-766X
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
322
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
455-62
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:16001266-Amino Acid Sequence,
pubmed-meshheading:16001266-Animals,
pubmed-meshheading:16001266-Cattle,
pubmed-meshheading:16001266-DNA,
pubmed-meshheading:16001266-Female,
pubmed-meshheading:16001266-Immunoblotting,
pubmed-meshheading:16001266-Interleukin-18,
pubmed-meshheading:16001266-Male,
pubmed-meshheading:16001266-Molecular Sequence Data,
pubmed-meshheading:16001266-Pituitary Gland, Anterior,
pubmed-meshheading:16001266-RNA, Messenger,
pubmed-meshheading:16001266-Receptors, Interleukin,
pubmed-meshheading:16001266-Receptors, Interleukin-18,
pubmed-meshheading:16001266-Reverse Transcriptase Polymerase Chain Reaction
|
pubmed:year |
2005
|
pubmed:articleTitle |
Localization of interleukin-18 and its receptor in somatotrophs of the bovine anterior pituitary gland.
|
pubmed:affiliation |
Laboratory of Functional Morphology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555, Sendai, Japan.
|
pubmed:publicationType |
Journal Article
|