Linked Life Data

15998290

Source:http://linkedlifedata.com/resource/pubmed/id/15998290

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-7-6
pubmed:abstractText
Cytokines and catecholamines are known to constitute a significant portion of the regulatory neuroimmune networks involved in maintaining homeostasis in the central nervous system (CNS). Although we have already reported an increase in norepinephrine (NE) turnover within the locus coeruleus (LC) at 2 and 4 h after the intraperitoneal (i.p.) injection of lipopolysaccharide (LPS), the implication of this increase remains unclear. In view of evidence that norepinephrine (NE) acts in an anti-inflammatory manner by way of negatively regulating pro-inflammatory cytokine expression, we examined the inflammatory cytokine expression levels in the LC of C3H/HeN mice (male, 8 weeks old) after an i.p. LPS injection. The mRNA expression levels of the genes encoding IL-1beta and TNF-alpha within the LC increased during the first 2 h, and showed two peaks, the first at 4 h and the second lesser one at 15 h after the LPS injection. Microglia, which are one of the major cell types that produce pro-inflammatory cytokines in the CNS, were isolated from mouse neonate brains in order to clarify more precisely the relationship between the changes in NE content and the up-regulation of inflammatory cytokines in the LC. Simultaneous incubation of microglia with LPS and NE enhanced the expression of IL-1beta at both mRNA and protein levels, but reduced the mRNA and protein levels of TNF-alpha. These data support the hypothesis that NE negatively regulates the expression of pro-inflammatory cytokine expression, at least in the case of TNF-alpha, which action could contribute to the observed anti-inflammatory properties of NE. This report, based on the results of both in vivo and in vitro experiments, is the first to suggest a relationship between NE content and cytokine expression levels in the CNS.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
94
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
393-404
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15998290-Analysis of Variance, pubmed-meshheading:15998290-Animals, pubmed-meshheading:15998290-Animals, Newborn, pubmed-meshheading:15998290-Caspase 1, pubmed-meshheading:15998290-Cells, Cultured, pubmed-meshheading:15998290-Cytokines, pubmed-meshheading:15998290-Drug Administration Routes, pubmed-meshheading:15998290-Drug Interactions, pubmed-meshheading:15998290-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:15998290-Gene Expression Regulation, pubmed-meshheading:15998290-Immunoblotting, pubmed-meshheading:15998290-Immunohistochemistry, pubmed-meshheading:15998290-Immunoprecipitation, pubmed-meshheading:15998290-Inflammation, pubmed-meshheading:15998290-Lipopolysaccharides, pubmed-meshheading:15998290-Locus Coeruleus, pubmed-meshheading:15998290-Male, pubmed-meshheading:15998290-Mice, pubmed-meshheading:15998290-Mice, Inbred C3H, pubmed-meshheading:15998290-Microglia, pubmed-meshheading:15998290-Norepinephrine, pubmed-meshheading:15998290-RNA, Messenger, pubmed-meshheading:15998290-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15998290-Time Factors
pubmed:year
2005
pubmed:articleTitle
Peripheral injection of lipopolysaccharide enhances expression of inflammatory cytokines in murine locus coeruleus: possible role of increased norepinephrine turnover.
pubmed:affiliation
Department of Physiology, Fujita Health University School of Medicine, Toyoake, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't