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rdf:type |
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lifeskim:mentions |
umls-concept:C0007585,
umls-concept:C0021368,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0078939,
umls-concept:C0205225,
umls-concept:C0206116,
umls-concept:C0243192,
umls-concept:C0441472,
umls-concept:C0754728,
umls-concept:C2349975
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pubmed:issue |
2
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pubmed:dateCreated |
2005-7-6
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pubmed:abstractText |
The interaction of endogenous and exogenous stimulators of innate immunity was examined in primary cultures of mouse microglial cells and macrophages after application of defined Toll-like receptor (TLR) agonists [lipopolysaccharide (LPS) (TLR4), the synthetic lipopeptide Pam3Cys-Ser-Lys4 (Pam3Cys) (TLR2) and single-stranded unmethylated CpG-DNA (CpG) (TLR9)] alone and in combination with amyloid beta peptide (Abeta) 1-40. Abeta1-40 stimulated microglial cells and macrophages primed by interferon-gamma in a dose-dependent manner. Co-administration of Abeta1-40 with LPS or Pam3Cys led to an additive release of nitric oxide (NO) and tumour necrosis factor alpha (TNF-alpha). This may be one reason for the clinical deterioration frequently observed in patients with Alzheimer's disease during infections. In contrast, co-application of Abeta1-40 with CpG led to a substantial decrease of NO and TNF-alpha release compared with stimulation with CpG alone. Abeta1-40 and CpG did not co-localize within the same subcellular compartment, making a direct physicochemical interaction as the cause of the observed antagonism very unlikely. This suggests that not all TLR agonists enhance the stimulatory effect of A beta on innate immunity.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amyloid beta-Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/CpG-DNA, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/N-palmitoyl-S-(2,3-bis(palmitoyloxy)...,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrites,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Tlr2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tlr4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tlr9 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptor 2,
http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptor 4,
http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptor 9,
http://linkedlifedata.com/resource/pubmed/chemical/amyloid beta-protein (1-40)
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-3042
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
94
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
289-98
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:15998280-Amyloid beta-Peptides,
pubmed-meshheading:15998280-Analysis of Variance,
pubmed-meshheading:15998280-Animals,
pubmed-meshheading:15998280-Animals, Newborn,
pubmed-meshheading:15998280-Blotting, Western,
pubmed-meshheading:15998280-Brain,
pubmed-meshheading:15998280-Cell Survival,
pubmed-meshheading:15998280-Cells, Cultured,
pubmed-meshheading:15998280-Cytokines,
pubmed-meshheading:15998280-DNA, Bacterial,
pubmed-meshheading:15998280-DNA-Binding Proteins,
pubmed-meshheading:15998280-Dose-Response Relationship, Drug,
pubmed-meshheading:15998280-Drug Interactions,
pubmed-meshheading:15998280-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:15998280-Immunohistochemistry,
pubmed-meshheading:15998280-Inflammation,
pubmed-meshheading:15998280-Lectins,
pubmed-meshheading:15998280-Lipopolysaccharides,
pubmed-meshheading:15998280-Lipoproteins,
pubmed-meshheading:15998280-Macrophages,
pubmed-meshheading:15998280-Mice,
pubmed-meshheading:15998280-Mice, Inbred C57BL,
pubmed-meshheading:15998280-Microglia,
pubmed-meshheading:15998280-Microscopy, Confocal,
pubmed-meshheading:15998280-Nitrites,
pubmed-meshheading:15998280-Peptide Fragments,
pubmed-meshheading:15998280-Receptors, Cell Surface,
pubmed-meshheading:15998280-Receptors, Immunologic,
pubmed-meshheading:15998280-Toll-Like Receptor 2,
pubmed-meshheading:15998280-Toll-Like Receptor 4,
pubmed-meshheading:15998280-Toll-Like Receptor 9
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pubmed:year |
2005
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pubmed:articleTitle |
Amyloid beta peptide 1-40 enhances the action of Toll-like receptor-2 and -4 agonists but antagonizes Toll-like receptor-9-induced inflammation in primary mouse microglial cell cultures.
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pubmed:affiliation |
Department of Neurology, Georg-August-University, Göttingen, Germany.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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