Source:http://linkedlifedata.com/resource/pubmed/id/15996230
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
2005-7-5
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| pubmed:abstractText |
The potential of porcine endogenous retrovirus (PERV) as a human pathogen, particularly as a public health risk, is a major concern for xenotransplantation. In vitroPERV transmission to human cells is well established. Evidence from human/pig hematopoietic chimeras in immunodeficient mice suggests PERV transmission from pig to human cells in vivo. However, recently Yang et al. demonstrated in such a model that PERV-C, a nonhuman-tropic class, could be transmitted via pseudotyping by xenotropic murine leukemia virus (X-MLV). We developed a mouse pig islet xenotransplant model, where pig and human cells are located in physically separate compartments, to directly assess PERV transmission from a functional pig xenograft. X-MLV efficiently pseudotypes all three classes of PERV, including PERV-A and -B that are known to productively infect human cell lines and PERV-C that is normally not infectious for human cells. Pseudotyping also extends PERV's natural tropism to nonpermissive, nonhuman primate cells. X-MLV is activated locally by the surgical procedure involved in the tissue transplants. Thus, the presence and activation of endogenous X-MLV in immunodeficient mice limits the clinical significance of previous reports of in vivo PERV transmission from pig tissues to human cells.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
1600-6135
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
5
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1837-47
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| pubmed:dateRevised |
2009-11-3
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| pubmed:meshHeading |
pubmed-meshheading:15996230-Amino Acid Sequence,
pubmed-meshheading:15996230-Animals,
pubmed-meshheading:15996230-Cells, Cultured,
pubmed-meshheading:15996230-DNA, Viral,
pubmed-meshheading:15996230-Endogenous Retroviruses,
pubmed-meshheading:15996230-Humans,
pubmed-meshheading:15996230-Islets of Langerhans,
pubmed-meshheading:15996230-Islets of Langerhans Transplantation,
pubmed-meshheading:15996230-Leukemia Virus, Murine,
pubmed-meshheading:15996230-Mice,
pubmed-meshheading:15996230-Mice, Inbred NOD,
pubmed-meshheading:15996230-Mice, SCID,
pubmed-meshheading:15996230-Models, Animal,
pubmed-meshheading:15996230-Molecular Sequence Data,
pubmed-meshheading:15996230-Retroviridae Infections,
pubmed-meshheading:15996230-Sequence Homology, Amino Acid,
pubmed-meshheading:15996230-Species Specificity,
pubmed-meshheading:15996230-Swine,
pubmed-meshheading:15996230-Transplantation, Heterologous,
pubmed-meshheading:15996230-Transplantation Chimera,
pubmed-meshheading:15996230-Virus Integration
|
| pubmed:year |
2005
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| pubmed:articleTitle |
Pseudotyping of porcine endogenous retrovirus by xenotropic murine leukemia virus in a pig islet xenotransplantation model.
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| pubmed:affiliation |
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|