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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2005-7-5
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pubmed:abstractText |
Herbal medicine has become an increasing popular therapeutic alternative among patients suffering from various inflammatory disorders. The Salvia miltiorrhizae water-soluble extract (SME) have been shown to possess antioxidant and anti-inflammatory properties in vitro. However, the mechanism of action and impact of SME on LPS-induced gene expression is still unknown. We report that SME significantly abrogated LPS-induced IkappaB phosphorylation/degradation, NF-kappaB transcriptional activity and ICAM-1 gene expression in rat IEC-18 cells. Chromatin immunoprecipitation assay demonstrated that LPS-induced RelA recruitment to the ICAM-1 gene promoter was inhibited by SME. Moreover, in vitro kinase assay showed that SME directly inhibits LPS induced IkappaB kinase (IKK) activity in IEC-18 cells. To investigate the physiological relevance of SME inhibitory activity on NF-kappaB signalling, we used small intestinal explants and primary intestinal epithelial cells derived from a transgenic mouse expressing the enhanced green fluorescent protein (EGFP) under the transcriptional control of NF-kappaB cis-elements (cis-NF-kappaB(EGFP)). SME significantly blocked LPS-induced EGFP expression and IkappaBalpha phosphorylation in intestinal explants and primary IECs, respectively. However, salvianolic acid B, an activate component of SME did not inhibit NF-kappaB transcriptional activity and IkappaB phosphorylation/degradation in IEC-18 cells. These results indicate that SME blocks LPS-induced NF-kappaB signalling pathway by targeting the IKK complex in intestinal epithelial cells. Modulation of bacterial product-mediated NF-kappaB signalling by natural plant extracts may represent an attractive strategy towards the prevention and treatment of intestinal inflammation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-10477620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-10488062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-10623846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-10679399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-10712233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-10721914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-10774465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-10961593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-11500927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-11999122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-12140289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-12222659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-12490687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-12499583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-12524386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-12672795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-12876555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-14630641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-14708018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-14734871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-14984374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-15016988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-15047038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-15093615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-15147574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-15179032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-15212763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-15265883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-15300204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-15479686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-15494486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-1739402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-2717690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-7972116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-8739039,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15996193-9833914
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Benzofurans,
http://linkedlifedata.com/resource/pubmed/chemical/Drugs, Chinese Herbal,
http://linkedlifedata.com/resource/pubmed/chemical/I-kappa B Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappaB inhibitor alpha,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Water,
http://linkedlifedata.com/resource/pubmed/chemical/salvianolic acid B
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
141
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
288-97
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15996193-Animals,
pubmed-meshheading:15996193-Mice,
pubmed-meshheading:15996193-Water,
pubmed-meshheading:15996193-Rats,
pubmed-meshheading:15996193-Intestinal Mucosa,
pubmed-meshheading:15996193-Lipopolysaccharides,
pubmed-meshheading:15996193-Phosphorylation,
pubmed-meshheading:15996193-Epithelial Cells,
pubmed-meshheading:15996193-Benzofurans,
pubmed-meshheading:15996193-Cells, Cultured,
pubmed-meshheading:15996193-RNA, Messenger,
pubmed-meshheading:15996193-Solubility,
pubmed-meshheading:15996193-Mice, Inbred BALB C,
pubmed-meshheading:15996193-Translocation, Genetic,
pubmed-meshheading:15996193-Gene Expression Regulation,
pubmed-meshheading:15996193-Signal Transduction,
pubmed-meshheading:15996193-Drugs, Chinese Herbal,
pubmed-meshheading:15996193-Salvia miltiorrhiza,
pubmed-meshheading:15996193-Mice, Transgenic,
pubmed-meshheading:15996193-NF-kappa B,
pubmed-meshheading:15996193-Intercellular Adhesion Molecule-1,
pubmed-meshheading:15996193-I-kappa B Proteins,
pubmed-meshheading:15996193-Reverse Transcriptase Polymerase Chain Reaction
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