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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
34
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pubmed:dateCreated |
2005-8-22
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pubmed:abstractText |
Laminin-5-rich extracellular matrix derived from 804G cells (804G-ECM) engages beta1 integrins to induce spreading, improve glucose-stimulated insulin secretion (GSIS), and increase survival of pancreatic beta cells. The present study examines whether 804G-ECM activates the transcriptional activity of NF-kappaB and the involvement of NF-kappaB in those effects of 804G-ECM on pancreatic beta cells. 804G-ECM induces nuclear translocation and the DNA binding activity of the p65 subunit of NF-kappaB. 804G-ECM-induced nuclear translocation of NF-kappaB was weak as compared with that induced by interleukin-1beta. Transient 804G-ECM-induced DNA binding activity of NF-kappaB (peak at 2 h) and overexpression of NF-kappaB target genes IkappaB alpha and NF-kappaB1(p105) (peak at 4 h) were observed. When NF-kappaB was inhibited by an inhibitor of IkappaB alpha phosphorylation (Bay 11-7082) or by a recombinant adenovirus expressing the nonphosphorylatable form of IkappaB alpha, 804G-ECM-induced cell spreading and actin cytoskeleton organization were reduced. GSIS from cells on 804G-ECM was inhibited 5-fold, whereas cell survival was not affected. In summary, the results indicate that 804G-ECM induces a transient and moderate NF-kappaB activity. This study shows for the first time that ECM-induced NF-kappaB activity is necessary in maintaining GSIS, although it does not affect survival of pancreatic beta cells. The effects of ECM-induced NF-kappaB activity contrast with the deleterious effects of cytokine-induced NF-kappaB activity. It is proposed that transient and moderate NF-kappaB activity is essential for proper function of the pancreatic beta cell.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3-(4-methylphenylsulfonyl)-2-propene...,
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD29,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/I kappa B beta protein,
http://linkedlifedata.com/resource/pubmed/chemical/I-kappa B Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappaB inhibitor alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Nitriles,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfones,
http://linkedlifedata.com/resource/pubmed/chemical/kalinin
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
280
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
30630-7
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:15994334-Actins,
pubmed-meshheading:15994334-Active Transport, Cell Nucleus,
pubmed-meshheading:15994334-Adenoviridae,
pubmed-meshheading:15994334-Animals,
pubmed-meshheading:15994334-Antigens, CD29,
pubmed-meshheading:15994334-Blotting, Western,
pubmed-meshheading:15994334-Cell Adhesion Molecules,
pubmed-meshheading:15994334-Cell Survival,
pubmed-meshheading:15994334-Cells, Cultured,
pubmed-meshheading:15994334-Cytoskeleton,
pubmed-meshheading:15994334-DNA,
pubmed-meshheading:15994334-Enzyme Inhibitors,
pubmed-meshheading:15994334-Extracellular Matrix,
pubmed-meshheading:15994334-Glucose,
pubmed-meshheading:15994334-I-kappa B Proteins,
pubmed-meshheading:15994334-In Situ Nick-End Labeling,
pubmed-meshheading:15994334-Insulin,
pubmed-meshheading:15994334-Islets of Langerhans,
pubmed-meshheading:15994334-Kinetics,
pubmed-meshheading:15994334-Male,
pubmed-meshheading:15994334-NF-kappa B,
pubmed-meshheading:15994334-Nitriles,
pubmed-meshheading:15994334-Phosphorylation,
pubmed-meshheading:15994334-Protein Binding,
pubmed-meshheading:15994334-Rats,
pubmed-meshheading:15994334-Rats, Wistar,
pubmed-meshheading:15994334-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15994334-Sulfones,
pubmed-meshheading:15994334-Time Factors
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pubmed:year |
2005
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pubmed:articleTitle |
Activation of NF-kappaB by extracellular matrix is involved in spreading and glucose-stimulated insulin secretion of pancreatic beta cells.
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pubmed:affiliation |
Department of Genetic Medicine and Development, University Medical Center, University Hospital, 1211 Geneva 4, Switzerland. eva.hammar@medecine.unige.ch
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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