| pubmed-article:15993097 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15993097 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:15993097 | lifeskim:mentions | umls-concept:C0011065 | lld:lifeskim |
| pubmed-article:15993097 | lifeskim:mentions | umls-concept:C1135918 | lld:lifeskim |
| pubmed-article:15993097 | lifeskim:mentions | umls-concept:C0003483 | lld:lifeskim |
| pubmed-article:15993097 | lifeskim:mentions | umls-concept:C1456820 | lld:lifeskim |
| pubmed-article:15993097 | lifeskim:mentions | umls-concept:C1704259 | lld:lifeskim |
| pubmed-article:15993097 | lifeskim:mentions | umls-concept:C1705987 | lld:lifeskim |
| pubmed-article:15993097 | lifeskim:mentions | umls-concept:C1515877 | lld:lifeskim |
| pubmed-article:15993097 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:15993097 | lifeskim:mentions | umls-concept:C0150312 | lld:lifeskim |
| pubmed-article:15993097 | lifeskim:mentions | umls-concept:C0049920 | lld:lifeskim |
| pubmed-article:15993097 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:15993097 | pubmed:dateCreated | 2005-7-4 | lld:pubmed |
| pubmed-article:15993097 | pubmed:abstractText | This study was undertaken to investigate whether a physiologically compatible concentration of 7-ketocholesterol had any effect on human vascular smooth muscle cells (HVSMCs). We found that 7-ketocholesterol changed the viability of human aorta smooth muscle cells (HAoSMC) not by cytotoxicity but by activation of tumor necrosis factor-alpha receptor (TNFR)-mediated death. Whereas TNF-alpha did not affect the viability in the presence of 7alpha-hydroxycholesterol or cholesterol, the cytokine induced HAoSMC death in the presence of 7-ketocholesterol as detected by morphology, viability, and fragmentation of chromosomal DNA. The HAoSMC death was inhibited by a neutralizing anti-TNF receptor 1 (TNFR1) antibody and by the caspase inhibitors of z-VAD and z-DEVD. Activations of caspase-8 and -3 were detected from dying HAoSMCs. 7-Ketocholesterol inhibited translocation of the nuclear factor kappaB (NF-kappaB) subunits of p65 and p50 from the cytosol into the nucleus, increase of NF-kappaB activity, and expression of caspase-8 homolog Fas ligand interleukin-1-converting enzyme inhibitory protein by TNF-alpha. We also found that X-chromosome-linked inhibitor of apoptosis protein was degraded in dying HAoSMC. The present study proposes that 7-ketocholesterol would contribute to the disappearance of HVSMC in the atherosclerotic lesions by enhancing receptor-mediated death. This is the first report demonstrating induction of TNF-alpha-mediated death by oxysterol in cells. | lld:pubmed |
| pubmed-article:15993097 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15993097 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15993097 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15993097 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15993097 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15993097 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15993097 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15993097 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:15993097 | pubmed:issn | 0006-291X | lld:pubmed |
| pubmed-article:15993097 | pubmed:author | pubmed-author:RhoMun-ChualM... | lld:pubmed |
| pubmed-article:15993097 | pubmed:author | pubmed-author:LeeHyun SunHS | lld:pubmed |
| pubmed-article:15993097 | pubmed:author | pubmed-author:RhimByung... | lld:pubmed |
| pubmed-article:15993097 | pubmed:author | pubmed-author:ChungMi... | lld:pubmed |
| pubmed-article:15993097 | pubmed:author | pubmed-author:KimYoung... | lld:pubmed |
| pubmed-article:15993097 | pubmed:author | pubmed-author:KimKoanhoiK | lld:pubmed |
| pubmed-article:15993097 | pubmed:author | pubmed-author:BaekJin AhJA | lld:pubmed |
| pubmed-article:15993097 | pubmed:author | pubmed-author:LeeHan... | lld:pubmed |
| pubmed-article:15993097 | pubmed:author | pubmed-author:ChangJong... | lld:pubmed |
| pubmed-article:15993097 | pubmed:author | pubmed-author:SokDai EunDE | lld:pubmed |
| pubmed-article:15993097 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15993097 | pubmed:day | 12 | lld:pubmed |
| pubmed-article:15993097 | pubmed:volume | 333 | lld:pubmed |
| pubmed-article:15993097 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15993097 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15993097 | pubmed:pagination | 1093-9 | lld:pubmed |
| pubmed-article:15993097 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:15993097 | pubmed:meshHeading | pubmed-meshheading:15993097... | lld:pubmed |
| pubmed-article:15993097 | pubmed:meshHeading | pubmed-meshheading:15993097... | lld:pubmed |
| pubmed-article:15993097 | pubmed:meshHeading | pubmed-meshheading:15993097... | lld:pubmed |
| pubmed-article:15993097 | pubmed:meshHeading | pubmed-meshheading:15993097... | lld:pubmed |
| pubmed-article:15993097 | pubmed:meshHeading | pubmed-meshheading:15993097... | lld:pubmed |
| pubmed-article:15993097 | pubmed:meshHeading | pubmed-meshheading:15993097... | lld:pubmed |
| pubmed-article:15993097 | pubmed:meshHeading | pubmed-meshheading:15993097... | lld:pubmed |
| pubmed-article:15993097 | pubmed:meshHeading | pubmed-meshheading:15993097... | lld:pubmed |
| pubmed-article:15993097 | pubmed:meshHeading | pubmed-meshheading:15993097... | lld:pubmed |
| pubmed-article:15993097 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15993097 | pubmed:articleTitle | TNF-alpha activates death pathway in human aorta smooth muscle cell in the presence of 7-ketocholesterol. | lld:pubmed |
| pubmed-article:15993097 | pubmed:affiliation | Laboratory of Lipid Metabolism, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea. | lld:pubmed |
| pubmed-article:15993097 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15993097 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:7124 | entrezgene:pubmed | pubmed-article:15993097 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:15993097 | lld:entrezgene |
