Source:http://linkedlifedata.com/resource/pubmed/id/15992380
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2005-7-21
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| pubmed:abstractText |
The endocannabinoid anandamide (N-arachidonoylethanolamine) and other bioactive long-chain N-acylethanolamines are thought to be formed from their corresponding N-acylphosphatidylethanolamines by a specific phospholipase D (NAPE-PLD) in the brain as well as other tissues. However, regional distribution of NAPE-PLD in the brain has not been examined. In the present study, we investigated the expression levels of NAPE-PLD in nine different regions of rat brain by enzyme assay, western blotting and real-time PCR. The NAPE-PLD activity was detected in all the tested brain regions with the highest activity in thalamus. Similar distribution patterns of NAPE-PLD were observed at protein and mRNA levels. We also found a remarkable increase in the expression levels of protein and mRNA of the brain NAPE-PLD with development, which was in good agreement with the increase in the activity. The age-dependent increase was also seen with several brain regions and other NAPE-PLD-enriched organs (heart and testis). p-Chloromercuribenzoic acid and cetyltrimethylammonium chloride, which inhibited recombinant NAPE-PLD dose-dependently, strongly inhibited the enzyme of all the brain regions. These results demonstrated wide distribution of NAPE-PLD in various brain regions and its age-dependent expression, suggesting the central role of this enzyme in the formation of anandamide and other N-acylethanolamines in the brain.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cetrimonium Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylethanolamines,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipase D,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/cetrimonium,
http://linkedlifedata.com/resource/pubmed/chemical/p-Chloromercuribenzoic Acid
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0022-3042
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
94
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
753-62
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15992380-Age Factors,
pubmed-meshheading:15992380-Aging,
pubmed-meshheading:15992380-Animals,
pubmed-meshheading:15992380-Animals, Newborn,
pubmed-meshheading:15992380-Blotting, Western,
pubmed-meshheading:15992380-Brain,
pubmed-meshheading:15992380-Brain Chemistry,
pubmed-meshheading:15992380-COS Cells,
pubmed-meshheading:15992380-Cercopithecus aethiops,
pubmed-meshheading:15992380-Cetrimonium Compounds,
pubmed-meshheading:15992380-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:15992380-Male,
pubmed-meshheading:15992380-Phosphatidylethanolamines,
pubmed-meshheading:15992380-Phospholipase D,
pubmed-meshheading:15992380-Rats,
pubmed-meshheading:15992380-Recombinant Proteins,
pubmed-meshheading:15992380-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15992380-Subcellular Fractions,
pubmed-meshheading:15992380-p-Chloromercuribenzoic Acid
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| pubmed:year |
2005
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| pubmed:articleTitle |
Regional distribution and age-dependent expression of N-acylphosphatidylethanolamine-hydrolyzing phospholipase D in rat brain.
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| pubmed:affiliation |
Department of Biochemistry, Kagawa University School of Medicine, Kagawa, Japan.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|