Linked Life Data

15990109

Source:http://linkedlifedata.com/resource/pubmed/id/15990109

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2005-7-15
pubmed:abstractText
Current flow cytometric technology allows quantitative assessment of surface and intracellularly expressed molecules on isolated cells. However, the need to disrupt tissues prevents correlation of phenotypic expression with anatomical location. In contrast, immunohistochemistry in conjunction with conventional or confocal microscopy allows localisation of staining, but little in the way of quantitation. The laser scanning cytometer (LSC) allows a combination of both approaches, as it can apply quantitative flow cytometric laser technology to intact tissue. The purpose of this protocol is to describe in vitro and ex vivo methods for quantifying cell signaling molecule expression and activation within antigen-specific T cells by laser scanning cytometry.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:volume
301
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
140-53
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Direct quantitation of T cell signaling by laser scanning cytometry.
pubmed:affiliation
Division of Immunology, Infection and Inflammation, Western Infirmary, University of Glasgow, Glasgow, G11 6NT, UK. amm43w@clinmed.gla.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't