Linked Life Data

15989271

Source:http://linkedlifedata.com/resource/pubmed/id/15989271

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2005-7-1
pubmed:abstractText
To explore the integrated bio-control mechanism of Trichoderma harzianum and acquire some bio-control associated novel genes, a Trichoderma harzianum mycelium cDNA library has been constructed and thereby randomly selected clones were sequenced and analyzed by bioinformatics analysis. Full-length cDNA, encoding class v chitinase (ChiV), successfully cloned. The results indicated that the ORF of ChiV was 1194 bp, encoding 397 aa, deduced molecular weight 44 kD. The gene was ligated to the vector of pYES2 and thereby transformed to the yeast of H158 species. The enzyme activity reached the peak expression after cultured for 60 h induced by beta-galactose. The optimal temperature of ChiV is 37 degrees C and the optimal pH is 6 and 8.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0001-6209
pubmed:author
pubmed:issnType
Print
pubmed:volume
45
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
253-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
[Cloning and expression in yeasts of the class V chitinase cDNA gene from Trichoderma harzianum].
pubmed:affiliation
Department of Life Science and Engineering, Harbin Inst of Technology, Harbin 150001, China. lpgkevin@sohu.com
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't