Linked Life Data

15987840

Source:http://linkedlifedata.com/resource/pubmed/id/15987840

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2005-6-30
pubmed:abstractText
Long-chain fatty acid and sn-2-monoacylglycerol (2-MG) are the digestive products of dietary triacylglycerol (TG) hydrolysis. Although fatty acid uptake into the enterocyte has been examined widely, less is known about 2-MG uptake, and few studies have mimicked the physiologic conditions present in the postprandial situation. In this study, the cellular uptake of oleic acid and 2-monoolein, presented in taurocholate micellar solution, was examined in human intestinal Caco-2 cells to model the postprandial intestinal milieu. Initial uptake of oleic acid and 2-MG displayed a saturable function of their monomer concentrations, suggesting that fatty acid and 2-MG uptake may be protein-mediated processes at low unbound concentrations of lipid. The initial rate of oleate uptake was faster and the apparent Km was lower than values for 2-MG. Unlabeled oleic acid and, to a lesser extent, unlabeled 2-MG, inhibited the uptakes of both [3H]oleic acid and [3H]2-monoolein, suggesting competitive uptake. The nonphysiologic isomer sn-1-MG had effects similar to 2-MG, whereas the intermediate digestive product, diacylglycerol (DG), did not inhibit either oleate or 2-monoolein uptake. These results suggest that in the postprandial state, fatty acid and 2-MG derived from dietary TG are transported into the enterocyte, at least in part, via a protein-mediated pathway that is shared by both lipids, but not by the intermediate digestive product, DG.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-3166
pubmed:author
pubmed:issnType
Print
pubmed:volume
135
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1626-30
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Uptake of micellar long-chain fatty acid and sn-2-monoacylglycerol into human intestinal Caco-2 cells exhibits characteristics of protein-mediated transport.
pubmed:affiliation
Department of Nutritional Sciences, Cook College, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural