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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
2005-7-15
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pubmed:abstractText |
High voltage-activated Ca(2+) channel expression and gating is controlled by their beta subunits. Although the sites of interaction are known at the atomic level, how beta modulates gating remains to be determined. Using a chimeric approach, beta subunit regulation was conferred to a low voltage-activated channel. Regulation was dependent on a rigid linker connecting the alpha(1) interaction domain to IS6. Chimeric channels also revealed a role for IS6 in channel gating. Taken together, these results support a direct coupling model where beta subunits alter movements in IS6 that occur as the channel transits between closed, open, and inactivated states.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
579
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3907-12
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:15987636-Amino Acid Sequence,
pubmed-meshheading:15987636-Animals,
pubmed-meshheading:15987636-Biophysical Phenomena,
pubmed-meshheading:15987636-Biophysics,
pubmed-meshheading:15987636-Calcium,
pubmed-meshheading:15987636-Calcium Channels,
pubmed-meshheading:15987636-Calcium Channels, N-Type,
pubmed-meshheading:15987636-Calcium Channels, T-Type,
pubmed-meshheading:15987636-Cell Line,
pubmed-meshheading:15987636-Circular Dichroism,
pubmed-meshheading:15987636-Electrophysiology,
pubmed-meshheading:15987636-Green Fluorescent Proteins,
pubmed-meshheading:15987636-Humans,
pubmed-meshheading:15987636-Kinetics,
pubmed-meshheading:15987636-Molecular Sequence Data,
pubmed-meshheading:15987636-Protein Structure, Tertiary,
pubmed-meshheading:15987636-Rats,
pubmed-meshheading:15987636-Recombinant Fusion Proteins,
pubmed-meshheading:15987636-Sequence Homology, Amino Acid,
pubmed-meshheading:15987636-Structure-Activity Relationship
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pubmed:year |
2005
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pubmed:articleTitle |
Transfer of beta subunit regulation from high to low voltage-gated Ca2+ channels.
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pubmed:affiliation |
Department of Pharmacology, University of Virginia, Charlottesville, VA 22908, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, N.I.H., Extramural
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