Source:http://linkedlifedata.com/resource/pubmed/id/15984157
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2005-6-29
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| pubmed:abstractText |
The objective of this study was to determine developmental pattern, total cell number, apoptosis and apoptosis-related gene expression in haploid and diploid embryos following parthenogenetic activation. In vitro-matured porcine oocytes were activated by electrical pulses and cultured in the absence or presence of cytochalasin B for 3 h. Zygotes with two polar bodies (haploid) and one polar body (diploid) were carefully selected and were further cultured in NCSU 23 medium containing 0.4% bovine serum albumin (BSA) for 7 days. The percentage of development to blastocyst stage was higher (p < 0.01) in the diploid than in the haploid parthenotes. In haploid blastocysts, average total cell number was significantly reduced (p < 0.05) and apoptosis was increased at day 7. The relative abundance of Bcl-xL and Bak mRNA in the diploid blastocysts was similar to that of in vivo-fertilized embryos. However, Bcl-xL was significantly decreased, and Bak mRNA was significantly increased (p < 0.05) in haploid parthenotes compared with the diploid parthenotes. These results suggest that the haploid state affects apoptosis-related gene expression which results in increased apoptosis and decreased developmental competence of haploid parthenotes.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-2 Homologous Antagonist-Killer...,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-X Protein
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
0967-1994
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
13
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
17-21
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15984157-Animals,
pubmed-meshheading:15984157-Apoptosis,
pubmed-meshheading:15984157-Blastocyst,
pubmed-meshheading:15984157-Cell Count,
pubmed-meshheading:15984157-Diploidy,
pubmed-meshheading:15984157-Embryo, Mammalian,
pubmed-meshheading:15984157-Embryo Culture Techniques,
pubmed-meshheading:15984157-Female,
pubmed-meshheading:15984157-Fertilization in Vitro,
pubmed-meshheading:15984157-Gene Expression Regulation, Developmental,
pubmed-meshheading:15984157-Haploidy,
pubmed-meshheading:15984157-Male,
pubmed-meshheading:15984157-Membrane Proteins,
pubmed-meshheading:15984157-Parthenogenesis,
pubmed-meshheading:15984157-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:15984157-RNA, Messenger,
pubmed-meshheading:15984157-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15984157-Swine,
pubmed-meshheading:15984157-Zygote,
pubmed-meshheading:15984157-bcl-2 Homologous Antagonist-Killer Protein,
pubmed-meshheading:15984157-bcl-X Protein
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| pubmed:year |
2005
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| pubmed:articleTitle |
Haploidy influences Bak and Bcl-xL mRNA expression and increases incidence of apoptosis in porcine embryos.
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| pubmed:affiliation |
Department of Animal Sciences, Research Center for Bioresource and Health, Cheongju, Chungbuk, South Korea.
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| pubmed:publicationType |
Journal Article
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