Linked Life Data

1598205

Source:http://linkedlifedata.com/resource/pubmed/id/1598205

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1992-7-9
pubmed:abstractText
The incorporation of fluorescently labeled dideoxynucleotides by T7 DNA polymerase is optimized by the use of Mn2+, fluorescein analogs and four 2'-deoxyribonucleoside 5'-O-(1-thiotriphosphates) (dNTP alpha S's). The one-tube extension protocol was tested on single-stranded templates, as well as PCR fragments which were made single-stranded by digestion with T7 gene 6 exonuclease. Dye primer sequencing using four dNTP alpha S's was shown to give uniform termination patterns which were comparable to four dNTPs. Efficiency of the polymerase also appeared to improve with the dNTP alpha S's. A mathematical model was developed to predict the pattern of termination based on enzyme activity and ratios of ddNTP/dNTPs. This method can be used to optimize sequencing reactions and to estimate enzyme discrimination constants of chain terminators.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1598205-1648367, http://linkedlifedata.com/resource/pubmed/commentcorrection/1598205-1835395, http://linkedlifedata.com/resource/pubmed/commentcorrection/1598205-1925562, http://linkedlifedata.com/resource/pubmed/commentcorrection/1598205-2159476, http://linkedlifedata.com/resource/pubmed/commentcorrection/1598205-2411211, http://linkedlifedata.com/resource/pubmed/commentcorrection/1598205-2443975, http://linkedlifedata.com/resource/pubmed/commentcorrection/1598205-2483818, http://linkedlifedata.com/resource/pubmed/commentcorrection/1598205-2587244, http://linkedlifedata.com/resource/pubmed/commentcorrection/1598205-2657738, http://linkedlifedata.com/resource/pubmed/commentcorrection/1598205-2703498, http://linkedlifedata.com/resource/pubmed/commentcorrection/1598205-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/1598205-3200828, http://linkedlifedata.com/resource/pubmed/commentcorrection/1598205-3323803, http://linkedlifedata.com/resource/pubmed/commentcorrection/1598205-3588303, http://linkedlifedata.com/resource/pubmed/commentcorrection/1598205-3713851, http://linkedlifedata.com/resource/pubmed/commentcorrection/1598205-3996185
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2471-83
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
DNA sequencing with dye-labeled terminators and T7 DNA polymerase: effect of dyes and dNTPs on incorporation of dye-terminators and probability analysis of termination fragments.
pubmed:affiliation
Applied Biosystems, Inc., Foster City, CA 94404.
pubmed:publicationType
Journal Article