Linked Life Data

15980607

Source:http://linkedlifedata.com/resource/pubmed/id/15980607

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2005-6-27
pubmed:abstractText
Amyloid-enhancing factor (AEF) is a biological "activity" that is defined in the context of inflammation-associated amyloidogenesis (AA). When administered intravenously to mice followed by an inflammatory stimulus, such primed mice deposit substantial AA amyloid in spleen within 36-48 h. Since experimental induction of AEF is dependent on amyloidogenic protocols, and rapid AA amyloid induction is dependent on AEF, a strategy for AEF isolation is required to break into this circular process. AEF activity may be prepared from a variety of human forms of amyloid that include tissue containing any of Abeta, AA, ATTR, and AL amyloids. The preparation of an AEF extract from such human tissue is described using 4 M glycerol, which then may be used to induce splenic AA amyloid fibrils in mice as a source for the propagation of additional AEF and/or for the study of amyloidogenesis. The glycerol and AA fibril preparations are stable frozen for many years.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
299
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
237-41
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2005
pubmed:articleTitle
Preparation and propagation of amyloid-enhancing factor.
pubmed:affiliation
Department of Pathology and Molecular Medicine, Queen's University and The Syl and Molly Apps Research Center, Kingston General Hospital, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't